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PMID: 14730021 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

P-site tRNA is a crucial initiator of ribosomal frameshifting.

RNA (New York, N.Y.) ·Vol. 10 ·No. 2 ·2004-02-00 ·Pages 221-30

Baranov PV, Gesteland RF, Atkins JF

Abstract

The expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed frameshifting. The present work extends this to other cases. When the A-site is unoccupied, the P-site tRNA can be repositioned forward with respect to mRNA (although repositioning in the minus direction is also possible). A kinetic model is presented for the influence of both, the cognate tRNAs competing for overlapping codons in A-site, and the stabilities of P-site tRNA:mRNA complexes in the initial and new frames. When the A-site is occupied, the P-site tRNA can be repositioned backward. Whether frameshifting will happen depends on the ability of the A-site tRNA to subsequently be repositioned to maintain physical proximity of the tRNAs. This model offers an alternative explanation to previously published mechanisms of programmed frameshifting, such as out-of-frame tRNA binding, and a different perspective on simultaneous tandem tRNA slippage.

MeSH Terms
Animals Anticodon/metabolism Codon/metabolism Frameshifting, Ribosomal/physiology Humans RNA, Transfer/metabolism Reading Frames/physiology Ribosomes/metabolism
Chemicals
Anticodon Codon RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baranov Pavel V
Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112-5330, USA.
Gesteland Raymond F
Atkins John F
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.5122604
Published
2004-02-00
Pages
221-30
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370534
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048152 · United States
NIGMS NIH HHS · R01-GM48152 · United States
NIGMS NIH HHS · R01-GM61200 · United States
External Links
PubMed source DOI: 10.1261/rna.5122604 PMC Full Text View journal details
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