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PMID: 11233984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Programmed +1 frameshifting stimulated by complementarity between a downstream mRNA sequence and an error-correcting region of rRNA.

RNA (New York, N.Y.) ·Vol. 7 ·No. 2 ·2001-02-00 ·Pages 275-84

Li Z, Stahl G, Farabaugh PJ

Abstract

Like most retroviruses and retrotransposons, the retrotransposon Ty3 expresses its pol gene analog (POL3) as a translational fusion to the upstream gag analog (GAG3). The Gag3-Pol3 fusion occurs by frameshifting during translation of the mRNA that encodes the two separate but overlapping ORFs. We showed previously that the shift occurs by out-of-frame binding of a normal aminoacyl-tRNA in the ribosomal A site caused by an aberrant codonoanticodon interaction in the P site. This event is unlike all previously described programmed translational frameshifts because it does not require tRNA slippage between cognate or near-cognate codons in the mRNA. A sequence of 15 nt distal to the frameshift site stimulates frameshifting 7.5-fold. Here we show that the Ty3 stimulator acts as an unstructured region to stimulate frameshifting. Its function depends on strict spacing from the site of frameshifting. Finally, the stimulator increases frameshifting dependent on sense codon-induced pausing, but has no effect on frameshifting dependent on pauses induced by nonsense codons. Complementarity between the stimulator and a portion of the accuracy center of the ribosome, Helix 18, implies that the stimulator may directly disrupt error correction by the ribosome.

MeSH Terms
Amino Acid Sequence Base Sequence Frameshifting, Ribosomal Fusion Proteins, gag-pol/genetics,metabolism Models, Molecular Molecular Sequence Data Mutation, Missense Nucleic Acid Conformation Plasmids Protein Biosynthesis RNA Viruses/physiology RNA, Messenger/chemistry,genetics,metabolism RNA, Ribosomal/chemistry,genetics,metabolism Retroelements/genetics Ribosomes/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Fusion Proteins, gag-pol RNA, Messenger RNA, Ribosomal Retroelements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li Z
Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore 21250, USA.
Stahl G
Farabaugh P J
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s135583820100190x
Published
2001-02-00
Pages
275-84
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370085
Subset
IM
Grants
NIGMS NIH HHS · GM 29480 · United States
FIC NIH HHS · TW02211-01 · United States
External Links
PubMed source DOI: 10.1017/s135583820100190x PMC Full Text View journal details
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