Home LiteratureArticle Details
PMID: 2163764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nascent peptide is required for ribosomal bypass of the coding gap in bacteriophage T4 gene 60.

Cell ·Vol. 62 ·No. 1 ·1990-07-13 ·Pages 117-26

Weiss RB, Huang WM, Dunn DM

Abstract

Bacteriophage T4 DNA topoisomerase gene 60 contains a 50 nucleotide untranslated region within the coding sequence of its mRNA. Translational bypass of this sequence by elongating ribosomes has been postulated for the mode of synthesis of an 18 kd polypeptide specified by the split coding segments. Ribosome bypass of the untranslated region also occurs when a segment of gene 60 is fused to lacZ and expressed in E. coli. The efficiency of bypass in these gene 60-lacZ fusions approaches 100%. Here, mutations that delete, insert, or substitute nucleotides from gene 60-lacZ fusions are examined. Essential features necessary for high level gap bypass emerging from this analysis are a cis-acting nascent peptide sequence, a short duplication bordering the gap, and a stop codon contained in a stem-loop structure at the 5' junction of the gap.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular Codon/genetics DNA Topoisomerases, Type II/genetics DNA Transposable Elements Escherichia coli/genetics Genes, Viral Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Protein Biosynthesis RNA, Messenger/genetics Recombinant Fusion Proteins/metabolism Ribosomes/metabolism T-Phages/enzymology,genetics Viral Structural Proteins/genetics beta-Galactosidase/genetics,metabolism
Chemicals
Codon DNA Transposable Elements RNA, Messenger Recombinant Fusion Proteins Viral Structural Proteins beta-Galactosidase DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Weiss R B
Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah.
Huang W M
Dunn D M
References (23)
23 references, click to expand
  1. Nascent polypeptide chains emerge from the exit domain of the large ribosomal subunit: immune mapping of the nascent chain.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3111-5 PMID: 6808502
  2. Interaction of secreted nascent chains with surrounding membrane in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5922-5 PMID: 104295
  3. Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin.
    Nature. 1988 Aug 18;334(6183):580-5 PMID: 3405308
  4. Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites.
    Biochim Biophys Acta. 1970 Dec 14;224(2):477-86 PMID: 5498079
  5. tRNAi(met) functions in directing the scanning ribosome to the start site of translation.
    Science. 1988 Oct 7;242(4875):93-7 PMID: 3051379
  6. T4 DNA-delay proteins, required for specific DNA replication, form a complex that has ATP-dependent DNA topoisomerase activity.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3737-41 PMID: 226976
  7. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7079-83 PMID: 6938954
  8. tRNA hopping: enhancement by an expanded anticodon.
    EMBO J. 1989 Dec 20;8(13):4315-23 PMID: 2686986
  9. T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication.
    Nature. 1979 Oct 11;281(5731):456-61 PMID: 226889
  10. Migration of 40 S ribosomal subunits on messenger RNA in the presence of edeine.
    J Biol Chem. 1978 Sep 25;253(18):6568-77 PMID: 681367
  11. Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region.
    Cell. 1988 Nov 4;55(3):447-58 PMID: 2846182
  12. Reading frame switch caused by base-pair formation between the 3' end of 16S rRNA and the mRNA during elongation of protein synthesis in Escherichia coli.
    EMBO J. 1988 May;7(5):1503-7 PMID: 2457498
  13. Slippery runs, shifty stops, backward steps, and forward hops: -2, -1, +1, +2, +5, and +6 ribosomal frameshifting.
    Cold Spring Harb Symp Quant Biol. 1987;52:687-93 PMID: 3135981
  14. Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance.
    Nucleic Acids Res. 1980 Dec 20;8(24):6081-97 PMID: 6162157
  15. Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot.
    Cell. 1989 May 19;57(4):537-47 PMID: 2720781
  16. The DNA-delay mutants of bacteriophage T4.
    J Virol. 1974 Oct;14(4):860-71 PMID: 4609406
  17. Rates of aminoacyl-tRNA selection at 29 sense codons in vivo.
    J Mol Biol. 1989 Sep 5;209(1):65-77 PMID: 2478714
  18. Ribosome pausing and stacking during translation of a eukaryotic mRNA.
    EMBO J. 1988 Nov;7(11):3559-69 PMID: 2850168
  19. A persistent untranslated sequence within bacteriophage T4 DNA topoisomerase gene 60.
    Science. 1988 Feb 26;239(4843):1005-12 PMID: 2830666
  20. Mutants of translational components that alter reading frame by two steps forward or one step back.
    J Biol Chem. 1988 Dec 5;263(34):18099-103 PMID: 2848024
  21. Interaction of antibiotics with functional sites in 16S ribosomal RNA.
    Nature. 1987 Jun 4-10;327(6121):389-94 PMID: 2953976
  22. Structure of ribosome-bound messenger RNA as revealed by enzymatic accessibility studies.
    J Mol Biol. 1985 Jan 20;181(2):241-51 PMID: 3845122
  23. CUUCGG hairpins: extraordinarily stable RNA secondary structures associated with various biochemical processes.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1364-8 PMID: 2449689
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(90)90245-a
Published
1990-07-13
Pages
117-26
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133334
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(90)90245-a PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2