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PMID: 1603058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active transcription from a promoter positioned within the coding region of a divergently oriented gene: the tobacco chloroplast rpl32 gene.

Molecular & general genetics : MGG ·Vol. 233 ·No. 1-2 ·1992-05-00 ·Pages 151-6

Vera A, Matsubayashi T, Sugiura M

Abstract

A new transcription unit has been identified and characterized in the small single-copy region of tobacco chloroplast DNA. A primary transcript (1550 nucleotides) spanning the entire transcription unit contains no significant open reading frames (ORFs), other than ORF55, recently identified as the gene encoding the ribosomal protein CL32 (rpl32). The leader sequence extends 1101 nucleotides from the rpl32 initiation codon. Primer extension and in vitro capping experiments in combination with ribonuclease protection assays, revealed a promoter situated more than 322 bp inside the coding region of ndhF, which is divergently oriented with respect to rpl32. A canonical Pribnow-box is found just upstream of the transcription start site, but a typical -35 motif was not detected. This is the first internal divergent promoter to be characterized in the chloroplast genome.

Related Genes
ndhF rpl32
MeSH Terms
Autoradiography Base Sequence Blotting, Northern Chloroplasts Molecular Sequence Data Nucleic Acid Hybridization Open Reading Frames Plant Proteins/genetics Plants, Toxic Promoter Regions, Genetic Ribosomal Proteins/genetics Tobacco/genetics Transcription, Genetic
Chemicals
Plant Proteins Ribosomal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vera A
Center for Gene Research, Nagoya University, Japan.
Matsubayashi T
Sugiura M
References (24)
24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00587573
Published
1992-05-00
Pages
151-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00587573
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