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PMID: 1708380 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli.

Journal of bacteriology ·Vol. 173 ·No. 9 ·1991-05-00 ·Pages 2864-71

Wu J, Weiss B

Abstract

soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requires both soxR and a new gene, soxS. soxS is adjacent to soxR, it encodes a 13-kDa protein, and it is required for paraquat resistance. These functions were revealed by studies in which the sequence of the 1.1-kb soxR-soxS region was determined, the 5' ends of the mRNAs were mapped, and complementation tests were performed with soxRS plasmids containing deletions of known sequence. The two genes are divergently transcribed, and the transcripts overlap. The soxS promoter is within the 85-nucleotide intergenic region, whereas the soxR promoter is within soxS. soxS mRNA increases after induction. Both protein products have possible DNA-binding (helix-turn-helix) domains. SoxR contains four cysteines (CX2CXCX5C) that might be part of a sensor region. SoxS shows 17 to 31% homology to the C-terminal portions of members of the AraC family of positive regulators.

Related Genes
AraC soxR soxS
MeSH Terms
Amino Acid Sequence Base Sequence Escherichia coli/drug effects,genetics Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Genetic Complementation Test Molecular Sequence Data Nucleotide Mapping Paraquat RNA/chemistry Sequence Homology, Nucleic Acid Superoxides/pharmacology Transcription, Genetic
Chemicals
Superoxides RNA Paraquat
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wu J
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
Weiss B
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46 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.9.2864-2871.1991
Published
1991-05-00
Pages
2864-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207867
Subset
IM
Grants
NCRR NIH HHS · MO1 RR 00042 · United States
Databases
GENBANK
M55488, M55489, M55490, M55491, M55492, M55493, M55494, M60111, M65246, M93064
External Links
PubMed source DOI: 10.1128/jb.173.9.2864-2871.1991 PMC Full Text View journal details
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