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PMID: 780339 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1082-8

White BJ, Hochhauser SJ, Cintron NM, Weiss B

Abstract

The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.

MeSH Terms
6-Aminonicotinamide/pharmacology Chromosome Mapping Deoxyribonucleases/biosynthesis Drug Resistance, Microbial Endonucleases/metabolism Escherichia coli/drug effects,enzymology Exonucleases/biosynthesis Genes Transduction, Genetic p-Fluorophenylalanine/pharmacology
Chemicals
6-Aminonicotinamide p-Fluorophenylalanine Deoxyribonucleases Endonucleases Exonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
White B J
Hochhauser S J
Cintron N M
Weiss B
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1082-1088.1976
Published
1976-06-00
Pages
1082-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233128
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1082-1088.1976 PMC Full Text View journal details
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