Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R.
The nucleotide sequence of the central part of Tn10 has been determined from the rightmost HindIII site to IS10R. This sequence contains two open reading frames with opposite polarity. The in vivo transcription start points in this sequence have been determined by S1 mapping. These results define one minor and two major promoters. The transcription starts of the two major promoters are only 18 base pairs apart, and the transcripts show different polarity and overlap by 18 base pairs. The nucleotide sequence reveals two regions with palindromic symmetry which may serve as operators. Their possible involvement in the regulation of transcription of both genes is discussed. Taken together these results allow for a maximal coding capacity of 138 amino acids directed toward IS10R and 197 amino acids directed toward tetA. The possible function of these gene products is discussed. The accompanying article (Braus et al., J. Bacteriol. 160:504-509, 1984) presents evidence that these genes are expressed.
28 references, click to expand
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Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome.J Bacteriol. 1975 Dec;124(3):1153-8 PMID: 1104574
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Characterization of a mini-ColC1 plasmid.J Bacteriol. 1976 Apr;126(1):447-53 PMID: 770430
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Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated.Proc Natl Acad Sci U S A. 1976 May;73(5):1509-12 PMID: 775491
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Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance.J Bacteriol. 1979 Jan;137(1):681-5 PMID: 762025
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The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.Adv Cyclic Nucleotide Res. 1979;10:135-67 PMID: 36738
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Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.J Bacteriol. 1979 Jun;138(3):705-14 PMID: 378932
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Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
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Identification of a second tetracycline-inducible polypeptide encoded by Tn10.J Bacteriol. 1980 Oct;144(1):346-55 PMID: 6252196
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Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974-7 PMID: 7001450
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Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli.J Gen Microbiol. 1980 Nov;121(1):221-9 PMID: 7019387
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Identification of the tetracycline resistance promoter and repressor in transposon Tn10.J Bacteriol. 1981 Aug;147(2):297-304 PMID: 6267006
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Preparation of milligram amounts of 21 deoxyribonucleic acid restriction fragments.Biochemistry. 1981 Jun 23;20(13):3748-56 PMID: 6268143
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Evidence for two functional gal promoters in intact Escherichia coli cells.J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
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pUR222, a vector for cloning and rapid chemical sequencing of DNA.Nucleic Acids Res. 1981 Aug 25;9(16):4087-98 PMID: 6272193
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Reduced expression of Tn 10-mediated tetracycline resistance in Escherichia coli containing more than one copy of the transposon.J Gen Microbiol. 1981 Sep;126(1):45-54 PMID: 6278056
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A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance.J Bacteriol. 1982 May;150(2):633-42 PMID: 6279565
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DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left.Proc Natl Acad Sci U S A. 1982 Apr;79(8):2608-12 PMID: 6283536
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Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.Nucleic Acids Res. 1983 Jan 25;11(2):525-39 PMID: 6298728
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A control element within a structural gene: the gal operon of Escherichia coli.Cell. 1983 Mar;32(3):783-8 PMID: 6299576
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Three promoters near the termini of IS10: pIN, pOUT, and pIII.Cell. 1983 Sep;34(2):673-82 PMID: 6311437
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Translational control of IS10 transposition.Cell. 1983 Sep;34(2):683-91 PMID: 6311438
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Overlapping divergent promoters control expression of Tn10 tetracycline resistance.Gene. 1983 Aug;23(2):149-56 PMID: 6311683
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Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.J Mol Biol. 1983 Sep 25;169(3):707-21 PMID: 6313933
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Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.Gene. 1983 Nov;25(1):83-92 PMID: 6319234
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Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region.J Mol Biol. 1984 Jan 15;172(2):185-201 PMID: 6229640
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Characterization of two mutations in the Escherichia coli galE gene inactivating the second galactose operator and comparative studies of repressor binding.EMBO J. 1983;2(12):2129-35 PMID: 6365532
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Identification of additional genes on transposon Tn10: tetC and tetD.J Bacteriol. 1984 Nov;160(2):504-9 PMID: 6094472
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