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PMID: 2460435 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the nfo gene of Escherichia coli K-12.

Journal of bacteriology ·Vol. 170 ·No. 11 ·1988-11-00 ·Pages 5141-5

Saporito SM, Cunningham RP

Abstract

The nfo gene of Escherichia coli K-12 which encodes endonuclease IV has been sequenced. The predicted gene product has a molecular weight of 31,562, in good agreement with the size of the gene product estimated by maxicell analysis. The nfo promoter was mapped by primer extension of in vivo transcripts. Inspection of the nucleotide sequence revealed no regions of potential secondary structure corresponding to a transcriptional terminator downstream from the structural gene; however, there was a potential open reading frame immediately downstream from the nfo structural gene.

MeSH Terms
Amino Acid Sequence Base Sequence DNA-(Apurinic or Apyrimidinic Site) Lyase Deoxyribonuclease IV (Phage T4-Induced) Endodeoxyribonucleases/genetics Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes Genes, Bacterial Molecular Sequence Data Molecular Weight Promoter Regions, Genetic Restriction Mapping
Chemicals
Escherichia coli Proteins Endodeoxyribonucleases Deoxyribonuclease IV (Phage T4-Induced) endonuclease IV, E coli DNA-(Apurinic or Apyrimidinic Site) Lyase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Saporito S M
Department of Biological Sciences, State University of New York, Albany 12222.
Cunningham R P
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.11.5141-5145.1988
Published
1988-11-00
Pages
5141-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211582
Subset
IM
Grants
NIGMS NIH HHS · GM33346 · United States
Databases
GENBANK
M22591
External Links
PubMed source DOI: 10.1128/jb.170.11.5141-5145.1988 PMC Full Text View journal details
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