Sequence and transcriptional analysis of the barley ctDNA region upstream of psbD-psbC encoding trnK(UUU), rps16, trnQ(UUG), psbK, psbI, and trnS(GCU).

Current genetics ·Vol. 17 ·No. 5 ·1990-05-00 ·Pages 445-54

Berends Sexton T, Jones JT, Mullet JE

Abstract

A 6.25 kbp barley plastid DNA region located between psbA and psbD-psbC were sequenced and RNAs produced from this DNA were analyzed. TrnK(UUU), rps16 and trnQ(UUG) were located upstream of psbA. These genes were transcribed from the same DNA strand as psbA and multiple RNAs hybridized to them. TrnK and rsp16 contained introns; a 504 amino acid open reading frame (ORF504) was located within the trnK intron. Between trnQ and psbD-psbC was a 2.24 kbp region encoding psbK, psbI and trnS(GCU). PsbK and psbI are encoded on the same DNA strand as psbD-psbC whereas trnS(GCU) is transcribed from the opposite strand. Two large RNAs accumulate in barley etioplasts which contain psbK, psbI, anti-sense trnS(GCU) and psbD-psbC sequences. Other RNAs encode psbK and psbI only, or psbK only. The divergent trnS(GCU) located upstream of psbD-psbC and a second divergent trnS(UGA) located downstream of psbD-psbC were both expressed. Furthermore, RNA complementary to psbK and psbI mRNA was detected, suggesting that transcription from divergent overlapping transcription units may modulate expression from this DNA region.

MeSH Terms

Amino Acid Sequence Base Sequence Blotting, Northern Chlorophyll/genetics Chloroplasts DNA/genetics Edible Grain/genetics Genes, Plant Hordeum/genetics Light-Harvesting Protein Complexes Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins Plant Proteins/genetics RNA/genetics Transcription, Genetic

Chemicals

Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Plant Proteins Chlorophyll RNA DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Berends Sexton T

M. D. Anderson Hospital, Department of Tumor Biology, Houston, TX 77030.

Jones J T

Mullet J E

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31 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00334526

Published

1990-05-00

Pages

445-54

Language

English

Region

United States

NLM ID

8004904

Subset

Databases

GENBANK

X52765

External Links

PubMed source DOI: 10.1007/BF00334526 View journal details

Analysis Services

Sequence and transcriptional analysis of the barley ctDNA region upstream of psbD-psbC encoding trnK(UUU), rps16, trnQ(UUG), psbK, psbI, and trnS(GCU).

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