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PMID: 1692772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The intron of the yeast actin gene contains the promoter for an antisense RNA.

Current genetics ·Vol. 17 ·No. 3 ·1990-03-00 ·Pages 269-73

Thompson-Jäger S, Domdey H

Abstract

Using Northern blot analysis we have detected an approximately 840 nucleotide-long RNA which is complementary to the 5' leader sequence and the first ten nucleotides of the coding sequence of the yeast actin (ACT1) messenger RNA. We have determined two transcription start sites for this actin antisense RNA (ASR1), both within the ACT1 intron, at about 80 and 90 nucleotides downstream from the 5' splice site. Analysis of a cDNA clone showed that this RNA species overlaps the entire trailer sequence and approximately 20 nucleotides of the coding sequence of the nearby yeast YPT1 gene.

MeSH Terms
Actins/genetics Base Sequence Blotting, Northern Fungal Proteins/genetics Genes, Fungal Introns Molecular Sequence Data Promoter Regions, Genetic RNA/genetics RNA, Antisense RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Actins Fungal Proteins RNA, Antisense RNA, Fungal RNA, Messenger RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thompson-Jäger S
Laboratorium für Molekulare Biologie-Genzentrum, Ludwig-Maximilians-Universität München, Martinsried, Federal Republic of Germany.
Domdey H
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00312620
Published
1990-03-00
Pages
269-73
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00312620 View journal details
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