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PMID: 1698606 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Three distinct ribonucleoproteins from tobacco chloroplasts: each contains a unique amino terminal acidic domain and two ribonucleoprotein consensus motifs.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3059-66

Li YQ, Sugiura M

Abstract

Chloroplasts contain their own genetic system. Eighteen different split genes have been found among approximately 130 chloroplast genes from higher plants. However, little is known about the chloroplast splicing system. Mammalian heterogeneous nuclear ribonucleoproteins (hnRNP proteins) have been shown to be involved in splicing. We applied a purification procedure developed for HeLa cell hnRNP proteins, which uses a single-stranded DNA (ssDNA) affinity column, directly to the tobacco chloroplast lysate to isolate their chloroplast counterparts. Four proteins (mol. wt approximately 30 kd) bound strongly to the column. The amino-terminal sequences of three of them were determined and their cDNA clones were isolated from a tobacco leaf cDNA library. Sequence analysis of these clones revealed that all three proteins contain two ribonucleoprotein consensus sequences (RNP-CS), confirming their ribonucleoprotein (RNP) nature. The presence of putative transit peptides in their predicted protein sequences, and an in vitro import experiment confirmed they are located in the chloroplast. This is the first report of organellar proteins containing RNP-CS. In addition, these three chloroplast proteins have a very acidic amino-terminal domain, a novel feature among RNP proteins identified so far. They are expressed both in leaves and roots; their mRNA levels showed different light modulation in mature leaves. The three proteins might be involved in splicing and/or processing of chloroplast RNAs.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Chloroplasts/metabolism Cloning, Molecular DNA/genetics,isolation & purification Gene Library Molecular Sequence Data Oligonucleotide Probes Plants, Toxic Protein Biosynthesis RNA/genetics,isolation & purification RNA Splicing RNA, Messenger/genetics Ribonucleoproteins/genetics Sequence Homology, Nucleic Acid Tobacco/genetics Transcription, Genetic
Chemicals
Oligonucleotide Probes RNA, Messenger Ribonucleoproteins RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Li Y Q
Center for Gene Research, Nagoya University, Japan.
Sugiura M
References (46)
46 references, click to expand
  1. Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
    Mol Cell Biol. 1988 May;8(5):2237-41 PMID: 3386636
  2. Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
    Mol Cell Biol. 1987 May;7(5):1731-9 PMID: 3110598
  3. The maize plastid psbB-psbF-petB-petD gene cluster: spliced and unspliced petB and petD RNAs encode alternative products.
    Curr Genet. 1987;12(1):69-77 PMID: 2835175
  4. Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
    EMBO J. 1989 Dec 20;8(13):4163-70 PMID: 2531658
  5. A binding consensus: RNA-protein interactions in splicing, snRNPs, and sex.
    Cell. 1989 Apr 7;57(1):1-3 PMID: 2522817
  6. Nucleotide sequences of cDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach.
    Curr Genet. 1988 Nov;14(5):511-8 PMID: 3066511
  7. Complete sequence of one of the mRNAs coding for the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris.
    Biochimie. 1984 Jul-Aug;66(7-8):539-45 PMID: 6549380
  8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  9. Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons.
    J Mol Biol. 1988 Apr 20;200(4):627-38 PMID: 3137346
  10. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
    Cell. 1989 Apr 7;57(1):89-101 PMID: 2467746
  11. Phenylalanines that are conserved among several RNA-binding proteins form part of a nucleic acid-binding pocket in the A1 heterogeneous nuclear ribonucleoprotein.
    J Biol Chem. 1988 Mar 5;263(7):3307-13 PMID: 2830282
  12. The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals.
    Mol Gen Genet. 1989 Jun;217(2-3):185-94 PMID: 2770692
  13. RNA binding specificity of hnRNP proteins: a subset bind to the 3' end of introns.
    EMBO J. 1988 Nov;7(11):3519-29 PMID: 3208740
  14. Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardii: evidence for in vivo trans-splicing.
    EMBO J. 1987 Aug;6(8):2185-95 PMID: 16453785
  15. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  16. High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4489-93 PMID: 3012569
  17. The transport of proteins into chloroplasts.
    Annu Rev Biochem. 1986;55:879-912 PMID: 3527053
  18. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.
    Nucleic Acids Res. 1974 Nov;1(11):1385-97 PMID: 10793698
  19. mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
    Mol Cell Biol. 1986 Aug;6(8):2932-43 PMID: 3537727
  20. RNP in maize protein.
    Nature. 1989 Jan 26;337(6205):312 PMID: 2521378
  21. Developmental, organ-specific, and light-dependent expression of the tomato ribulose-1,5-bisphosphate carboxylase small subunit gene family.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7104-8 PMID: 3478683
  22. Polymorphism of a photosystem I subunit caused by alloploidy in Nicotiana.
    Plant Physiol. 1990 Jan;92(1):273-5 PMID: 16667259
  23. In vitro synthesis and assembly of photosystem II proteins of spinach chloroplasts.
    Arch Biochem Biophys. 1986 Feb 1;244(2):517-27 PMID: 2418785
  24. A new and fast method for preparing high quality lambda DNA suitable for sequencing.
    Nucleic Acids Res. 1988 Apr 11;16(7):2873-84 PMID: 2966928
  25. The elav gene product of Drosophila, required in neurons, has three RNP consensus motifs.
    Science. 1988 Dec 16;242(4885):1570-2 PMID: 3144044
  26. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
    EMBO J. 1986 Sep;5(9):2043-2049 PMID: 16453699
  27. Heterogeneous nuclear ribonucleoprotein particles and the pathway of mRNA formation.
    Trends Biochem Sci. 1988 Mar;13(3):86-91 PMID: 3072706
  28. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein.
    Nature. 1988 Jul 21;334(6179):262-4 PMID: 2969461
  29. Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.
    Mol Cell Biol. 1989 Oct;9(10):4179-86 PMID: 2531275
  30. Oligonucleotide probes for the screening of recombinant DNA libraries.
    Methods Enzymol. 1987;152:432-42 PMID: 3309569
  31. Complex RNA maturation in chloroplasts. The psbB operon from spinach.
    Eur J Biochem. 1988 Feb 1;171(3):551-64 PMID: 2831053
  32. Heterogeneous nuclear ribonucleoproteins: role in RNA splicing.
    Science. 1986 Mar 28;231(4745):1534-9 PMID: 3952495
  33. Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein.
    Nature. 1989 Dec 14;342(6251):819-21 PMID: 2532307
  34. Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9788-92 PMID: 2557628
  35. Rapid splicing and stepwise processing of a transcript from the psbB operon in tobacco chloroplasts: determination of the intron sites in petB and petD.
    Mol Gen Genet. 1987 Oct;209(3):427-31 PMID: 17193705
  36. Large-scale purification of hnRNP proteins from HeLa cells by affinity chromatography on ssDNA-cellulose.
    Eur J Biochem. 1987 Jan 2;162(1):213-20 PMID: 3545821
  37. Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures.
    Biochem J. 1983 Jan 1;209(1):281-4 PMID: 6847617
  38. A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
    Cell. 1986 Jun 20;45(6):827-35 PMID: 3518950
  39. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  40. Isolation and characterization of a Xenopus laevis C protein cDNA: structure and expression of a heterogeneous nuclear ribonucleoprotein core protein.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9669-73 PMID: 2904678
  41. The chloroplast chromosomes in land plants.
    Annu Rev Cell Biol. 1989;5:51-70 PMID: 2688709
  42. Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.
    Genes Dev. 1988 Feb;2(2):215-27 PMID: 3129338
  43. RNA-binding proteins as developmental regulators.
    Genes Dev. 1989 Apr;3(4):431-7 PMID: 2470643
  44. cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms.
    Nucleic Acids Res. 1988 May 11;16(9):3751-70 PMID: 2836799
  45. The gene for the Mr 10,000 phosphoprotein associated with photosystem II is part of the psbB operon of the spinach plastid chromosome.
    Curr Genet. 1986;11(3):165-9 PMID: 2834086
  46. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
    Anal Biochem. 1984 Apr;138(1):141-3 PMID: 6731838
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3059-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552030
Subset
IM
Databases
GENBANK
X53932, X53933, X53942
External Links
PubMed source PMC Full Text View journal details
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