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PMID: 15831453 Published · ppublish English Journal Article

Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p.

Molecular and cellular biology ·Vol. 25 ·No. 9 ·2005-05-00 ·Pages 3461-74

Qiu H, Hu C, Zhang F, Hwang GJ, Swanson MJ, Boonchird C, Hinnebusch AG

Abstract

Transcriptional activation by Gcn4p is enhanced by the coactivators SWI/SNF, SAGA, and Srb mediator, which stimulate recruitment of TATA binding protein (TBP) and polymerase II to target promoters. We show that wild-type recruitment of SAGA by Gcn4p is dependent on mediator but independent of SWI/SNF function at three different promoters. Recruitment of mediator is also independent of SWI/SNF but is enhanced by SAGA at a subset of Gcn4p target genes. Recruitment of all three coactivators to ARG1 is independent of the TATA element and preinitiation complex formation, whereas efficient recruitment of the general transcription factors requires the TATA box. We propose an activation pathway involving interdependent recruitment of SAGA and Srb mediator to the upstream activation sequence, enabling SWI/SNF recruitment and the binding of TBP and other general factors to the promoter. We also found that high-level recruitment of Tra1p and other SAGA subunits is independent of the Ada2p/Ada3p/Gcn5p histone acetyltransferase module but requires Spt3p in addition to subunits required for SAGA integrity. Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo.

MeSH Terms
Acetyltransferases/physiology DNA-Binding Proteins/genetics,metabolism,physiology Gene Expression Regulation, Fungal/genetics,physiology Histone Acetyltransferases Promoter Regions, Genetic/genetics Protein Kinases/genetics,metabolism,physiology RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology TATA-Box Binding Protein/metabolism Trans-Activators/genetics,metabolism,physiology Transcription Factors/genetics,metabolism Transcription, Genetic/genetics,physiology
Chemicals
ADA2 protein, S cerevisiae DNA-Binding Proteins NGG1 protein, S cerevisiae SPT3 protein, S cerevisiae Saccharomyces cerevisiae Proteins TATA-Box Binding Protein TRA1 protein, S cerevisiae Trans-Activators Transcription Factors Acetyltransferases Histone Acetyltransferases Protein Kinases RNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Qiu Hongfang
Laboratory of Gene Regulation and Development, National Institute of Child Health & Human Development/NIH, Building 6A, Bethesda, MD 20892, USA.
Hu Cuihua
Zhang Fan
Hwang Gwo Jiunn
Swanson Mark J
Boonchird Cheunchit
Hinnebusch Alan G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.9.3461-3474.2005
Published
2005-05-00
Pages
3461-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1084306
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.25.9.3461-3474.2005 PMC Full Text View journal details
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