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PMID: 8413201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ADA3: a gene, identified by resistance to GAL4-VP16, with properties similar to and different from those of ADA2.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 5981-9

Piña B, Berger S, Marcus GA, Silverman N, Agapite J, Guarente L

Abstract

We describe the isolation of a yeast gene, ADA3, mutations in which prevent the toxicity of GAL4-VP16 in vivo. Toxicity was previously proposed to be due to the trapping of general transcription factors required at RNA polymerase II promoters (S. L. Berger, B. Piña, N. Silverman, G. A. Marcus, J. Agapite, J. L. Regier, S. J. Triezenberg, and L. Guarente, Cell 70:251-265, 1992). trans activation by VP16 as well as the acidic activation domain of GCN4 is reduced in the mutant. Other activation domains, such as those of GAL4 and HAP4, are only slightly affected in the mutant. This spectrum is similar to that observed for mutants with lesions in ADA2, a gene proposed to encode a transcriptional adaptor. The ADA3 gene is not absolutely essential for cell growth, but gene disruption mutants grow slowly and are temperature sensitive. Strains doubly disrupted for ada2 and ada3 grow no more slowly than single mutants, providing further evidence that these genes function in the same pathway. Selection of initiation sites by the general transcriptional machinery in vitro is altered in the ada3 mutant, providing a clue that ADA3 could be a novel general transcription factor involved in the response to acidic activators.

Related Genes
ADA2 ADA3
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal Drug Resistance, Microbial/genetics Fungal Proteins/genetics,pharmacology Genes, Fungal Molecular Sequence Data Mutation Promoter Regions, Genetic Saccharomyces cerevisiae/drug effects,genetics,growth & development Saccharomyces cerevisiae Proteins Trans-Activators/pharmacology Transcription Factors/genetics Transcription, Genetic Transcriptional Activation
Chemicals
DNA, Fungal Fungal Proteins Gal-VP16 NGG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Piña B
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Berger S
Marcus G A
Silverman N
Agapite J
Guarente L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.5981-5989.1993
Published
1993-10-00
Pages
5981-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364647
Subset
IM
Grants
NIGMS NIH HHS · GM30454 · United States
Databases
GENBANK
L21189
External Links
PubMed source DOI: 10.1128/mcb.13.10.5981-5989.1993 PMC Full Text View journal details
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