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PMID: 11562354 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II.

Genes & development ·Vol. 15 ·No. 18 ·2001-09-15 ·Pages 2457-69

Bhoite LT, Yu Y, Stillman DJ

Abstract

Regulation of HO gene expression in the yeast Saccharomyces cerevisiae is intricately orchestrated by an assortment of gene-specific DNA-binding and non-DNA binding regulators. Binding of the early G1 transcription factor Swi5 to the distal URS1 element of the HO promoter initiates a cascade of events through recruitment of the Swi/Snf and SAGA complexes. In late G1, binding of transcription factor SBF to promoter proximal sequences results in the timely expression of HO. In this work we describe an important additional layer of complexity to the current model by identifying a connection between Swi5 and the Mediator/RNA polymerase II holoenzyme complex. We show that Swi5 recruits Mediator to HO by specific interaction with the Gal11 module of the Mediator complex. Importantly, binding of both the Gal11 and Srb4 mediator components to the upstream region of HO is independent of the SBF factor. Swi/Snf is required for Mediator binding, and genetic suppression experiments suggest that Swi/Snf and Mediator act in the same genetic pathway of HO activation. Experiments examining the kinetics of binding show that Mediator binds to HO promoter elements 1.5 kb upstream of the transcription start site in early G1, but this binding occurs without RNA Pol II. RNA Pol II does not bind to HO until late G1, when HO is actively transcribed, and binding occurs exclusively to the TATA region.

MeSH Terms
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins/genetics,metabolism,physiology Gene Expression Regulation, Fungal Mediator Complex Promoter Regions, Genetic Protein Binding RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins TATA Box Trans-Activators/metabolism Transcription Factors/metabolism,physiology Transcription, Genetic
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins GAL11 protein, S cerevisiae Mediator Complex SRB4 protein, S cerevisiae SWI5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bhoite L T
Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA.
Yu Y
Stillman D J
References (64)
64 references, click to expand
  1. Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays.
    Mol Cell. 1999 Oct;4(4):649-55 PMID: 10549297
  2. An activator target in the RNA polymerase II holoenzyme.
    Mol Cell. 1998 May;1(6):895-904 PMID: 9660972
  3. Architectural transcription factors and the SAGA complex function in parallel pathways to activate transcription.
    Mol Cell Biol. 2000 Apr;20(7):2350-7 PMID: 10713159
  4. Genetic analysis of the YDR1-BUR6 repressor complex reveals an intricate balance among transcriptional regulatory proteins in yeast.
    Mol Cell Biol. 2000 Apr;20(7):2455-65 PMID: 10713169
  5. Transcriptional regulation through Mediator-like coactivators in yeast and metazoan cells.
    Trends Biochem Sci. 2000 Jun;25(6):277-83 PMID: 10838567
  6. A dual role for zinc fingers in both DNA binding and zinc sensing by the Zap1 transcriptional activator.
    EMBO J. 2000 Jul 17;19(14):3704-13 PMID: 10899124
  7. Mediator-nucleosome interaction.
    Mol Cell. 2000 Jul;6(1):197-201 PMID: 10949041
  8. Mediator of transcriptional regulation.
    Annu Rev Biochem. 2000;69:729-49 PMID: 10966474
  9. Dynamic association of capping enzymes with transcribing RNA polymerase II.
    Genes Dev. 2000 Oct 1;14(19):2435-40 PMID: 11018011
  10. In vivo requirement of activator-specific binding targets of mediator.
    Mol Cell Biol. 2000 Dec;20(23):8709-19 PMID: 11073972
  11. Functional connections between mediator components and general transcription factors of Saccharomyces cerevisiae.
    J Biol Chem. 2000 Nov 24;275(47):37251-6 PMID: 10973956
  12. A transcription reinitiation intermediate that is stabilized by activator.
    Nature. 2000 Nov 9;408(6809):225-9 PMID: 11089979
  13. Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter.
    Cell. 2000 Nov 10;103(4):667-78 PMID: 11106736
  14. Transcription of eukaryotic protein-coding genes.
    Annu Rev Genet. 2000;34:77-137 PMID: 11092823
  15. A genome-wide analysis of transcriptional effect of Gal11 in Saccharomyces cerevisiae: an application of "mini-array hybridization technique".
    DNA Res. 2001 Feb 28;8(1):23-31 PMID: 11258797
  16. Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p.
    Mol Microbiol. 2001 Apr;40(2):422-32 PMID: 11309124
  17. Cdk1 triggers association of RNA polymerase to cell cycle promoters only after recruitment of the mediator by SBF.
    Mol Cell. 2001 Jun;7(6):1213-20 PMID: 11430824
  18. Elevated recombination rates in transcriptionally active DNA.
    Cell. 1989 Feb 24;56(4):619-30 PMID: 2645056
  19. The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription.
    Mol Cell Biol. 1989 Dec;9(12):5602-9 PMID: 2685570
  20. Getting started with yeast.
    Methods Enzymol. 1991;194:3-21 PMID: 2005794
  21. New SNF genes, GAL11 and GRR1 affect SUC2 expression in Saccharomyces cerevisiae.
    Genetics. 1991 Nov;129(3):675-84 PMID: 1752413
  22. Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Oct;12(10):4503-14 PMID: 1406639
  23. TSF3, a global regulatory protein that silences transcription of yeast GAL genes, also mediates repression by alpha 2 repressor and is identical to SIN4.
    Mol Cell Biol. 1993 Feb;13(2):831-40 PMID: 8423805
  24. Mammalian Ras interacts directly with the serine/threonine kinase Raf.
    Cell. 1993 Jul 16;74(1):205-14 PMID: 8334704
  25. The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11237-41 PMID: 7902583
  26. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
    Cell. 1994 May 20;77(4):599-608 PMID: 8187178
  27. Positive and negative transcriptional regulation by the yeast GAL11 protein depends on the structure of the promoter and a combination of cis elements.
    Mol Gen Genet. 1994 Nov 1;245(3):301-12 PMID: 7816040
  28. Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast.
    Cell. 1995 Feb 24;80(4):583-92 PMID: 7867066
  29. Contact with a component of the polymerase II holoenzyme suffices for gene activation.
    Cell. 1995 May 5;81(3):359-68 PMID: 7736588
  30. General requirement for RNA polymerase II holoenzymes in vivo.
    Proc Natl Acad Sci U S A. 1995 May 9;92(10):4587-90 PMID: 7753848
  31. Association of an activator with an RNA polymerase II holoenzyme.
    Genes Dev. 1995 Apr 15;9(8):897-910 PMID: 7774808
  32. Regulation of HIS4 expression by the Saccharomyces cerevisiae SIN4 transcriptional regulator.
    Genetics. 1995 May;140(1):103-14 PMID: 7635278
  33. Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
    Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10864-8 PMID: 7479899
  34. Determining the requirements for cooperative DNA binding by Swi5p and Pho2p (Grf10p/Bas2p) at the HO promoter.
    J Biol Chem. 1995 Dec 8;270(49):29151-61 PMID: 7493941
  35. SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II.
    Mol Cell Biol. 1996 Jan;16(1):115-20 PMID: 8524287
  36. Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure.
    Genetics. 1995 Nov;141(3):873-88 PMID: 8582633
  37. EGT2 gene transcription is induced predominantly by Swi5 in early G1.
    Mol Cell Biol. 1996 Jul;16(7):3264-74 PMID: 8668141
  38. Gene activation by recruitment of the RNA polymerase II holoenzyme.
    Genes Dev. 1996 Sep 15;10(18):2359-67 PMID: 8824594
  39. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast.
    Genetics. 1996 Dec;144(4):1425-36 PMID: 8978031
  40. Global alterations in chromatin accessibility associated with loss of SIN4 function.
    Nucleic Acids Res. 1997 Mar 15;25(6):1240-7 PMID: 9092635
  41. Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters.
    Cell. 1997 May 2;89(3):365-71 PMID: 9150136
  42. 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics.
    Yeast. 1997 Jun 15;13(7):647-53 PMID: 9200814
  43. A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators.
    Mol Cell Biol. 1997 Aug;17(8):4852-8 PMID: 9234741
  44. Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.
    Cell. 1997 Aug 22;90(4):649-60 PMID: 9288745
  45. Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes.
    Genetics. 1997 Oct;147(2):451-65 PMID: 9335585
  46. Transcriptional activators direct histone acetyltransferase complexes to nucleosomes.
    Nature. 1998 Jul 30;394(6692):498-502 PMID: 9697775
  47. Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription.
    Mol Cell Biol. 1998 Sep;18(9):5364-70 PMID: 9710620
  48. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.
    Yeast. 1998 Jul;14(10):953-61 PMID: 9717241
  49. Residues in the Swi5 zinc finger protein that mediate cooperative DNA binding with the Pho2 homeodomain protein.
    Mol Cell Biol. 1998 Nov;18(11):6436-46 PMID: 9774660
  50. Dissecting the regulatory circuitry of a eukaryotic genome.
    Cell. 1998 Nov 25;95(5):717-28 PMID: 9845373
  51. Mediator protein mutations that selectively abolish activated transcription.
    Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):67-72 PMID: 9874773
  52. Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation.
    Mol Cell Biol. 1999 Feb;19(2):979-88 PMID: 9891034
  53. A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling.
    Mol Cell Biol. 1999 Feb;19(2):1056-67 PMID: 9891041
  54. An activator binding module of yeast RNA polymerase II holoenzyme.
    Mol Cell Biol. 1999 Apr;19(4):2967-76 PMID: 10082564
  55. Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter.
    Cell. 1999 Apr 30;97(3):299-311 PMID: 10319811
  56. RNA polymerase II as a control panel for multiple coactivator complexes.
    Curr Opin Genet Dev. 1999 Apr;9(2):132-9 PMID: 10322136
  57. Cell cycle-regulated histone acetylation required for expression of the yeast HO gene.
    Genes Dev. 1999 Jun 1;13(11):1412-21 PMID: 10364158
  58. Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines.
    Yeast. 1999 Jul;15(10B):963-72 PMID: 10407276
  59. Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators.
    Genes Dev. 1999 Sep 15;13(18):2369-74 PMID: 10500094
  60. Yeast Gal11 and transcription factor IIE function through a common pathway in transcriptional regulation.
    J Biol Chem. 1997 Dec 19;272(51):32663-9 PMID: 9405484
  61. The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11.
    Genetics. 1997 Dec;147(4):1585-94 PMID: 9409823
  62. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.
    Genes Dev. 1998 Jan 1;12(1):45-54 PMID: 9420330
  63. The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complex.
    Mol Cell Biol. 1998 Mar;18(3):1711-24 PMID: 9488488
  64. Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator.
    Mol Cell. 1999 Oct;4(4):657-64 PMID: 10549298
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.921601
Published
2001-09-15
Pages
2457-69
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312787
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.921601 PMC Full Text View journal details
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