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PMID: 9874773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mediator protein mutations that selectively abolish activated transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 1 ·1999-01-05 ·Pages 67-72

Myers LC, Gustafsson CM, Hayashibara KC, Brown PO, Kornberg RD

Abstract

Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression profile of a Deltamed2 strain showed diminished transcription of a subset of inducible genes but only minor effects on "basal" transcription. These findings make an important connection between transcriptional activation in vitro and in vivo, and identify Mediator as a "global" transcriptional coactivator.

MeSH Terms
Cell-Free System DNA-Binding Proteins Fungal Proteins/metabolism Gene Deletion Gene Expression Regulation, Fungal Models, Genetic Mutation Protein Kinases/metabolism Saccharomyces cerevisiae Proteins Structure-Activity Relationship Trans-Activators/genetics,metabolism Transcription, Genetic Transcriptional Activation Yeasts/genetics
Chemicals
DNA-Binding Proteins Fungal Proteins Gal-VP16 Saccharomyces cerevisiae Proteins Trans-Activators Protein Kinases carboxy-terminal domain kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Myers L C
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Gustafsson C M
Hayashibara K C
Brown P O
Kornberg R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.1.67
Published
1999-01-05
Pages
67-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC15094
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036659 · United States
NIGMS NIH HHS · R37 GM036659 · United States
NIGMS NIH HHS · GM36659 · United States
Corrections
CommentIn
PMID: 9874759
External Links
PubMed source DOI: 10.1073/pnas.96.1.67 PMC Full Text View journal details
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