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PMID: 8668141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

EGT2 gene transcription is induced predominantly by Swi5 in early G1.

Molecular and cellular biology ·Vol. 16 ·No. 7 ·1996-07-00 ·Pages 3264-74

Kovacech B, Nasmyth K, Schuster T

Abstract

In a screen for cell cycle-regulated genes in the yeast Saccharomyces cerevisiae, we have identified a gene, EGT2, which is involved in cell separation in the G1 stage of the cell cycle. Transcription of EGT2 is tightly regulated in a cell cycle-dependent manner. Transcriptional levels peak at the boundary of mitosis and early G1 The transcription factors responsible for EGT2 expression in early G1 are Swi5 and, to a lesser extent, Ace2. Swi5 is involved in the transcriptional activation of the HO gene during late G1 and early S phase, and Ace2 induces CTS1 transcription during early and late G1 We show that Swi5 activates EGT2 transcription as soon as it enters the nucleus at the end of mitosis in a concentration-dependent manner. Since Swi5 is unstable in the nucleus, its level drops rapidly, causing termination of EGT2 transcription before cells are committed to the next cell cycle. However, Swi5 is still able to activate transcription of HO in late G1 in conjunction with additional activators such as Swi4 and Swi6.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle/genetics Cell Cycle Proteins/biosynthesis,genetics Consensus Sequence DNA-Binding Proteins/metabolism Fungal Proteins/metabolism G1 Phase Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal Genotype Molecular Sequence Data Phenotype Promoter Regions, Genetic Protein Sorting Signals/biosynthesis,chemistry Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/metabolism Transcription, Genetic
Chemicals
Cell Cycle Proteins DNA-Binding Proteins EGT2 protein, S cerevisiae Fungal Proteins Protein Sorting Signals SWI5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kovacech B
Institute of Medical Radiation and Cell Research, University of Würzburg, Germany.
Nasmyth K
Schuster T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.7.3264
Published
1996-07-00
Pages
3264-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231320
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.7.3264 PMC Full Text View journal details
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