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PMID: 15385957 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II.

The EMBO journal ·Vol. 23 ·No. 20 ·2004-10-13 ·Pages 4040-50

Lemieux K, Gaudreau L

Abstract

The chromatin remodeling activity of the Swi/Snf complex is essential for the expression of several yeast genes. Previous studies have suggested that recruitment of Swi/Snf requires the action of transcriptional activators. However, reports in metazoans and in yeast have provided evidence of interactions between Swi/Snf and the RNA polymerase II holoenzyme/Mediator complex. Here we show that recruitment of Swi/Snf to the galactose-inducible gene GAL1 cannot be fully achieved without the integrity of the Mediator complex, TAF IIs, and RNA polymerase II. Moreover, artificial recruitment of Mediator is sufficient to tether both Swi/Snf and SAGA to the GAL1 UAS G. We further demonstrate that Swi/Snf recruitment at GAL1 does not require acetylation of chromatin by Gcn5 nor the presence of SAGA. Based on these results, we conclude that interactions between the Gal4 activator and Swi/Snf are not sufficient to recruit the latter to the GAL1 UAS G, since interactions with the Mediator, TAF IIs, and RNA polymerase II are also important.

MeSH Terms
Chromatin/metabolism Fungal Proteins/genetics,metabolism Genes, Fungal Nuclear Proteins/genetics,metabolism Precipitin Tests Promoter Regions, Genetic RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism TATA-Binding Protein Associated Factors/metabolism Trans-Activators/genetics,metabolism Transcription Factor TFIID
Chemicals
Chromatin Fungal Proteins Nuclear Proteins SWI3 protein, S cerevisiae Saccharomyces cerevisiae Proteins TAF2 protein, S cerevisiae TATA-Binding Protein Associated Factors Trans-Activators Transcription Factor TFIID RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lemieux Karine
Département de Biologie, Faculté des Sciences, Centre de Recherche sur les Mécanismes du Fonctionnement Cellulaire, Université de Sherbrooke, Sherbrooke, Québec, Canada.
Gaudreau Luc
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600416
Published
2004-10-13
Epub
2004-00-23
Pages
4040-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC524348
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600416 PMC Full Text View journal details
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