Home LiteratureArticle Details
PMID: 12941702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

H2A.Z has a function reminiscent of an activator required for preferential binding to intergenic DNA.

The EMBO journal ·Vol. 22 ·No. 17 ·2003-09-01 ·Pages 4512-22

Larochelle M, Gaudreau L

Abstract

H2A.Z has been shown to regulate transcription in yeast, and that function resides in its C-terminal region as the reciprocal portion of H2A cannot substitute for the latter. We show that fusion of a transcriptional activating region to the C-terminal region of H2A, which is substituted for that of H2A.Z, can allow the chimera to fulfil the special role of H2A.Z in positive gene regulation, as well as complement growth deficiencies of htz1delta cells. We further show that the 'transcription' function of H2A.Z is linked to its ability to preferentially localize to certain intergenic DNA regions. Our results suggest that H2A.Z modulates functional interactions with transcription regulatory components, and thus increases its localization to promoters where it helps poise chromatin for gene activation.

MeSH Terms
DNA, Fungal/metabolism DNA, Intergenic/metabolism Gene Expression Regulation, Fungal Genes, Fungal Histones/metabolism Hydroxyurea/metabolism Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/metabolism Transcriptional Activation
Chemicals
DNA, Fungal DNA, Intergenic Histones Saccharomyces cerevisiae Proteins Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Larochelle Marc
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1, Canada.
Gaudreau Luc
References (52)
52 references, click to expand
  1. ATP-dependent chromatin remodeling activities.
    Cell Mol Life Sci. 2001 May;58(5-6):673-82 PMID: 11437229
  2. Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators.
    Genes Dev. 1999 Sep 15;13(18):2369-74 PMID: 10500094
  3. The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4.
    Genes Dev. 2001 Aug 1;15(15):1946-56 PMID: 11485989
  4. H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions.
    Mol Cell Biol. 2001 Sep;21(18):6270-9 PMID: 11509669
  5. Histone variant H2A.Z is required for early mammalian development.
    Curr Biol. 2001 Aug 7;11(15):1183-7 PMID: 11516949
  6. Histone acetyltransferases.
    Annu Rev Biochem. 2001;70:81-120 PMID: 11395403
  7. The essential histone variant H2A.Z regulates the equilibrium between different chromatin conformational states.
    Nat Struct Biol. 2002 Mar;9(3):172-6 PMID: 11850638
  8. ATP-dependent nucleosome remodeling.
    Annu Rev Biochem. 2002;71:247-73 PMID: 12045097
  9. A target essential for the activity of a nonacidic yeast transcriptional activator.
    Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8591-6 PMID: 12084920
  10. The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
    Mol Cell. 2002 Jun;9(6):1191-200 PMID: 12086617
  11. Requirement of yeast RAD2, a homolog of human XPG gene, for efficient RNA polymerase II transcription. implications for Cockayne syndrome.
    Cell. 2002 Jun 28;109(7):823-34 PMID: 12110180
  12. A conserved RING finger protein required for histone H2B monoubiquitination and cell size control.
    Mol Cell. 2003 Jan;11(1):261-6 PMID: 12535538
  13. Conserved histone variant H2A.Z protects euchromatin from the ectopic spread of silent heterochromatin.
    Cell. 2003 Mar 7;112(5):725-36 PMID: 12628191
  14. Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II.
    Mol Cell Biol. 2003 Jun;23(12):4207-18 PMID: 12773564
  15. H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2819-23 PMID: 6574451
  16. Deletion analysis of GAL4 defines two transcriptional activating segments.
    Cell. 1987 Mar 13;48(5):847-53 PMID: 3028647
  17. Characterization of a cDNA clone coding for a sea urchin histone H2A variant related to the H2A.F/Z histone protein in vertebrates.
    Nucleic Acids Res. 1987 Jun 11;15(11):4629-44 PMID: 2438657
  18. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80.
    Cell. 1987 Jul 3;50(1):137-42 PMID: 3297349
  19. Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays.
    Mol Cell. 1999 Oct;4(4):649-55 PMID: 10549297
  20. ATP-dependent chromatin-remodeling complexes.
    Mol Cell Biol. 2000 Mar;20(6):1899-910 PMID: 10688638
  21. An artificial transcriptional activating region with unusual properties.
    Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):1988-92 PMID: 10681438
  22. Histone H2A.Z is widely but nonrandomly distributed in chromosomes of Drosophila melanogaster.
    J Biol Chem. 2000 Jul 28;275(30):23267-72 PMID: 10801889
  23. Saccharomyces cerevisiae transcription elongation mutants are defective in PUR5 induction in response to nucleotide depletion.
    Mol Cell Biol. 2000 Oct;20(20):7427-37 PMID: 11003640
  24. Global role for chromatin remodeling enzymes in mitotic gene expression.
    Cell. 2000 Sep 1;102(5):587-98 PMID: 11007477
  25. Yeast chromatin structure and regulation of GAL gene expression.
    Prog Nucleic Acid Res Mol Biol. 2001;65:197-259 PMID: 11008489
  26. Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.
    Cell. 2000 Oct 27;103(3):411-22 PMID: 11081628
  27. Global histone acetylation and deacetylation in yeast.
    Nature. 2000 Nov 23;408(6811):495-8 PMID: 11100734
  28. Crystal structure of a nucleosome core particle containing the variant histone H2A.Z.
    Nat Struct Biol. 2000 Dec;7(12):1121-4 PMID: 11101893
  29. A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR.
    Mol Cell. 2000 Oct;6(4):769-80 PMID: 11090616
  30. Gcn4 activator targets Gcn5 histone acetyltransferase to specific promoters independently of transcription.
    Mol Cell. 2000 Dec;6(6):1309-20 PMID: 11163205
  31. Transcriptional regulation of the yeast gmp synthesis pathway by its end products.
    J Biol Chem. 2001 Jan 12;276(2):1523-30 PMID: 11035032
  32. Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit.
    Science. 2001 Jun 22;292(5525):2333-7 PMID: 11423663
  33. Sequence and properties of the message encoding Tetrahymena hv1, a highly evolutionarily conserved histone H2A variant that is associated with active genes.
    Nucleic Acids Res. 1988 Jan 11;16(1):179-98 PMID: 3340523
  34. Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species.
    Nucleic Acids Res. 1988 Feb 11;16(3):1113-24 PMID: 3344202
  35. Drosophila has a single copy of the gene encoding a highly conserved histone H2A variant of the H2A.F/Z type.
    Nucleic Acids Res. 1988 Aug 11;16(15):7487-97 PMID: 3137528
  36. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae.
    Curr Genet. 1992 Jul;22(1):9-11 PMID: 1611672
  37. Temporal and spatial association of histone H2A variant hv1 with transcriptionally competent chromatin during nuclear development in Tetrahymena thermophila.
    Genes Dev. 1993 Dec;7(12B):2641-51 PMID: 8276246
  38. The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast.
    Genes Dev. 1994 Oct 15;8(20):2401-15 PMID: 7958905
  39. Analysis of a histone H2A variant from fission yeast: evidence for a role in chromosome stability.
    Mol Gen Genet. 1994 Dec 1;245(5):628-35 PMID: 7808414
  40. GAL4 interacts with TATA-binding protein and coactivators.
    Mol Cell Biol. 1995 May;15(5):2839-48 PMID: 7739564
  41. Quantitation of putative activator-target affinities predicts transcriptional activating potentials.
    EMBO J. 1996 Aug 1;15(15):3951-63 PMID: 8670900
  42. H2A.ZI, a new variant histone expressed during Xenopus early development exhibits several distinct features from the core histone H2A.
    Nucleic Acids Res. 1996 Oct 15;24(20):3947-52 PMID: 8918796
  43. A likely histone H2A.F/Z variant in Saccharomyces cerevisiae.
    Trends Biochem Sci. 1996 Dec;21(12):466-7 PMID: 9009827
  44. Transcription factors vs nucleosomes: regulation of the PHO5 promoter in yeast.
    Trends Biochem Sci. 1997 Mar;22(3):93-7 PMID: 9066259
  45. RNA polymerase II holoenzyme recruitment is sufficient to remodel chromatin at the yeast PHO5 promoter.
    Cell. 1997 Apr 4;89(1):55-62 PMID: 9094714
  46. Transcriptional activation by recruitment.
    Nature. 1997 Apr 10;386(6625):569-77 PMID: 9121580
  47. An activator target in the RNA polymerase II holoenzyme.
    Mol Cell. 1998 May;1(6):895-904 PMID: 9660972
  48. Activator-mediated recruitment of the RNA polymerase II machinery is the predominant mechanism for transcriptional activation in yeast.
    Mol Cell. 1998 May;1(6):917-24 PMID: 9660975
  49. Transcriptional activators direct histone acetyltransferase complexes to nucleosomes.
    Nature. 1998 Jul 30;394(6692):498-502 PMID: 9697775
  50. Recruitment of the RNA polymerase II holoenzyme and its implications in gene regulation.
    Biol Chem. 1998 Dec;379(12):1397-405 PMID: 9894806
  51. Regions of variant histone His2AvD required for Drosophila development.
    Nature. 1999 Jun 17;399(6737):694-7 PMID: 10385122
  52. SAGA is an essential in vivo target of the yeast acidic activator Gal4p.
    Genes Dev. 2001 Aug 1;15(15):1935-45 PMID: 11485988
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg427
Published
2003-09-01
Pages
4512-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC202369
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg427 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2