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PMID: 11081628 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.

Cell ·Vol. 103 ·No. 3 ·2000-10-27 ·Pages 411-22

Santisteban MS, Kalashnikova T, Smith MM

Abstract

Nucleosomes impose a block to transcription that can be overcome in vivo by remodeling complexes such as SNF/SWI and histone modification complexes such as SAGA. Mutations in the major core histones relieve transcriptional repression and bypass the requirement for SNF/SWI and SAGA. We have found that the variant histone H2A.Z regulates gene transcription, and deletion of the gene encoding H2A.Z strongly increases the requirement for SNF/SWI and SAGA. This synthetic genetic interaction is seen at the level of single genes and acts downstream of promoter nucleosome reorganization. H2A.Z is preferentially crosslinked in vivo to intergenic DNA at the PH05 and GAL1 loci, and this association changes with transcriptional activation. These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure.

MeSH Terms

Adenosine Triphosphatases Alleles Chromosomal Proteins, Non-Histone/genetics,physiology DNA, Fungal/genetics,metabolism DNA, Intergenic/genetics,metabolism DNA-Binding Proteins/genetics,metabolism,physiology Fungal Proteins/genetics,physiology Gene Deletion Gene Expression Regulation, Fungal Genes, Essential/genetics Genes, Fungal/genetics,physiology Histones/chemistry,genetics,metabolism Hot Temperature Macromolecular Substances Membrane Transport Proteins/genetics Molecular Conformation Nuclear Proteins Nucleosomes/chemistry,genetics,metabolism Phenotype Phosphate Transport Proteins Promoter Regions, Genetic/genetics Protein Binding Protein Kinases/genetics,physiology Protein Subunits Recombinant Fusion Proteins Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins Suppression, Genetic/genetics Transcription Factors/genetics,metabolism Transcription, Genetic

Chemicals

Chromosomal Proteins, Non-Histone DNA, Fungal DNA, Intergenic DNA-Binding Proteins Fungal Proteins Histones Macromolecular Substances Membrane Transport Proteins Nuclear Proteins Nucleosomes Phosphate Transport Proteins Protein Subunits Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors phosphate permease SPT2 protein, S cerevisiae Protein Kinases Adenosine Triphosphatases SNF2 protein, S cerevisiae

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Santisteban M S

Department of Microbiology and Cancer Center, University of Virginia, Charlottesville 22908, USA.

Kalashnikova T

Smith M M

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)00133-1

Published

2000-10-27

Pages

411-22

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NIGMS NIH HHS · GM28920 · United States

External Links

PubMed source DOI: 10.1016/s0092-8674(00)00133-1 View journal details

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Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.

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