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PMID: 15716492 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Optimizing the nucleotide sequence of a meiotic recombination hotspot in Schizosaccharomyces pombe.

Genetics ·Vol. 169 ·No. 4 ·2005-04-00 ·Pages 1973-83

Steiner WW, Smith GR

Abstract

The ade6-M26 mutation of Schizosaccharomyces pombe created a meiotic recombination hotspot. Previous analyses indicated that the heptamer 5'-ATGACGT-3' was necessary and sufficient for hotspot activity; the Atf1-Pcr1 transcription factor binds to this sequence and activates M26. After finding cases in which the M26 heptamer in ade6 was, surprisingly, not active as a hotspot, we used an in vitro selection method (SELEX) that revealed an 18-bp consensus sequence for Atf1-Pcr1 binding, 5'-GNVTATGACGTCATNBNC-3', containing the M26 heptamer at its core. Using this consensus sequence as a guide, we made mutations on each side of the heptamer at two separate sites in ade6. These mutations increased the intracellular hotspot activity of the heptamer, in some cases by >15-fold. These results show that M26, the eukaryotic recombination hotspot with the most precisely defined nucleotide sequence, is larger than previously thought, and they provide valuable information for clarifying the role of M26, and perhaps other hotspots, in meiotic recombination.

MeSH Terms
Alleles Base Sequence Binding Sites Chromosomes, Fungal Crosses, Genetic Gene Expression Regulation, Fungal Genes, Fungal Genetic Techniques Meiosis Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Mutation Polymerase Chain Reaction Recombination, Genetic Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins/genetics,physiology Sequence Homology, Nucleic Acid
Chemicals
Schizosaccharomyces pombe Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Steiner Walter W
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
Smith Gerald R
References (30)
30 references, click to expand
  1. A family of cAMP-response-element-related DNA sequences with meiotic recombination hotspot activity in Schizosaccharomyces pombe.
    Genetics. 2000 Sep;156(1):59-68 PMID: 10978275
  2. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.
    Microbiol Mol Biol Rev. 1999 Jun;63(2):349-404 PMID: 10357855
  3. The genetic control of meiosis.
    Annu Rev Genet. 1976;10:53-134 PMID: 797314
  4. A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity.
    Genes Dev. 1994 Jul 15;8(14):1693-702 PMID: 7958849
  5. Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans.
    Mol Cell Biol. 1995 Mar;15(3):1679-88 PMID: 7862159
  6. DNA motif associated with meiotic double-strand break regions in Saccharomyces cerevisiae.
    EMBO Rep. 2000 Sep;1(3):232-8 PMID: 11256605
  7. DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination host spot allele ade6-M26.
    J Mol Biol. 1988 Dec 20;204(4):917-25 PMID: 3221399
  8. Meiotic DNA breaks at the S. pombe recombination hot spot M26.
    Mol Cell. 2002 Apr;9(4):847-55 PMID: 11983175
  9. Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26.
    Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7446-51 PMID: 9207111
  10. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
    Science. 1990 Aug 3;249(4968):505-10 PMID: 2200121
  11. The strong ADH1 promoter stimulates mitotic and meiotic recombination at the ADE6 gene of Schizosaccharomyces pombe.
    Mol Cell Biol. 1991 Jan;11(1):289-98 PMID: 1986226
  12. Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6621-5 PMID: 8341678
  13. Meiotic recombination hot spots and cold spots.
    Nat Rev Genet. 2001 May;2(5):360-9 PMID: 11331902
  14. Site Specific Induction of Gene Conversion in SCHIZOSACCHAROMYCES POMBE.
    Genetics. 1971 Nov;69(3):317-37 PMID: 17248549
  15. A specific DNA sequence is required for high frequency of recombination in the ade6 gene of fission yeast.
    EMBO J. 1991 Aug;10(8):2157-63 PMID: 2065658
  16. Chromosomal context dependence of a eukaryotic recombinational hot spot.
    Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):227-31 PMID: 1729693
  17. A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe.
    Genetics. 2002 May;161(1):461-8 PMID: 12019258
  18. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe.
    Yeast. 1998 Jul;14(10):943-51 PMID: 9717240
  19. Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11383-90 PMID: 11027339
  20. Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13765-70 PMID: 9391101
  21. The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast.
    Genes Dev. 1997 Apr 1;11(7):876-86 PMID: 9106659
  22. Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
    Genetics. 1989 Sep;123(1):45-54 PMID: 2806887
  23. A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae.
    EMBO Rep. 2004 Jan;5(1):41-6 PMID: 14710185
  24. Conjugation, meiosis, and the osmotic stress response are regulated by Spc1 kinase through Atf1 transcription factor in fission yeast.
    Genes Dev. 1996 Sep 15;10(18):2276-88 PMID: 8824587
  25. Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain.
    Genetics. 1999 May;152(1):101-15 PMID: 10224246
  26. Clustering of meiotic double-strand breaks on yeast chromosome III.
    Proc Natl Acad Sci U S A. 1997 May 13;94(10):5213-8 PMID: 9144217
  27. Active and inactive transplacement of the M26 recombination hotspot in Schizosaccharomyces pombe.
    Genetics. 1995 Sep;141(1):33-48 PMID: 8536980
  28. Double-strand breaks at an initiation site for meiotic gene conversion.
    Nature. 1989 Mar 2;338(6210):87-90 PMID: 2645528
  29. Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes.
    Mol Cell Biol. 1999 Nov;19(11):7661-71 PMID: 10523654
  30. Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot.
    EMBO J. 2004 Apr 21;23(8):1792-803 PMID: 14988732
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.039230
Published
2005-04-00
Epub
2005-00-16
Pages
1973-83
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449614
Subset
IM
Grants
NIGMS NIH HHS · GM20190 · United States
NIGMS NIH HHS · GM31693 · United States
NIGMS NIH HHS · F32 GM020190 · United States
NIGMS NIH HHS · R01 GM031693 · United States
NIGMS NIH HHS · R56 GM031693 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.039230 PMC Full Text View journal details
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