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PMID: 15545655 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+.

Genetics ·Vol. 169 ·No. 3 ·2005-03-00 ·Pages 1243-60

Freeman-Cook LL, Gómez EB, Spedale EJ, Marlett J, Forsburg SL, Pillus L, Laurenson P

Abstract

In Schizosaccharomyces pombe, three genes, sir2(+), hst2(+), and hst4(+), encode members of the Sir2 family of conserved NAD(+)-dependent protein deacetylases. The S. pombe sir2(+) gene encodes a nuclear protein that is not essential for viability or for resistance to treatment with UV or a microtubule-destabilizing agent. However, sir2(+) is essential for full transcriptional silencing of centromeres, telomeres, and the cryptic mating-type loci. Chromatin immunoprecipitation results suggest that the Sir2 protein acts directly at these chromosomal regions. Enrichment of Sir2p at silenced regions does not require the HP1 homolog Swi6p; instead, Swi6-GFP localization to telomeres depends in part on Sir2p. The phenotype of sir2 swi6 double mutants supports a model whereby Sir2p functions prior to Swi6p at telomeres and the silent mating-type loci. However, Sir2p does not appear to be essential for the localization of Swi6p to centromeric foci. Cross-complementation experiments showed that the Saccharomyces cerevisiae SIR2 gene can function in place of S. pombe sir2(+), suggesting overlapping deacetylation substrates in both species. These results also suggest that, despite differences in most of the other molecules required, the two distantly related yeast species share a mechanism for targeting Sir2p homologs to silent chromatin.

MeSH Terms
Alternative Splicing Amino Acid Sequence Base Sequence Chromatin/genetics Conserved Sequence DNA Primers Exons Gene Expression Regulation, Fungal Gene Silencing Genes, Fungal Genes, Mating Type, Fungal Genes, Reporter Green Fluorescent Proteins/genetics Molecular Sequence Data Polymerase Chain Reaction Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins/genetics Sequence Alignment Sequence Homology, Amino Acid Sirtuins/genetics Transfection
Chemicals
Chromatin DNA Primers Schizosaccharomyces pombe Proteins Green Fluorescent Proteins Sirtuins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Freeman-Cook Lisa L
Division of Biological Sciences, Section of Molecular Biology and UCSD Center for Cancer Research, University of California, San Diego, 92093-0347, USA. lpillus@biomail.ucsd.edu
Gómez Eliana B
Spedale Erik J
Marlett John
Forsburg Susan L
Pillus Lorraine
Laurenson Patricia
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.032714
Email
lpillus@biomail.ucsd.edu
Published
2005-03-00
Epub
2004-00-15
Pages
1243-60
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449530
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059321-05A1 · United States
NIGMS NIH HHS · R01 GM059321 · United States
NIGMS NIH HHS · R01 GM054778 · United States
NIGMS NIH HHS · GM54778 · United States
NIGMS NIH HHS · GM59321 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.032714 PMC Full Text View journal details
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