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PMID: 1549592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Translational frameshifting mediated by a viral sequence in plant cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 6 ·1992-03-15 ·Pages 2262-6

Brault V, Miller WA

Abstract

It has been proposed that the polymerase gene of barley yellow dwarf virus and related viruses is expressed by a ribosomal frameshift event during translation. The 5' end of this gene overlaps with the 3' end of an upstream gene that is in a different reading frame. The region of overlap is similar to sequences in retro- and coronaviruses that are known to express their polymerase genes by frameshifting. This overlap region includes a "shifty" heptanucleotide, followed by a highly structured region that may contain a pseudoknot. Sequences of 115 or 144 base pairs that span this region from barley yellow dwarf virus (PAV serotype) genomic RNA were introduced into a plasmid, so that a reporter gene could be expressed in plant cells only if a minus one (-1) frameshift event occurred. Frameshifting was detected at a rate of approximately 1%. This frameshifting was abolished when the stop codon at the 3' end of the upstream open reading frame was deleted. A sequence expected to form a strong stem-loop immediately upstream of the frameshift site was unnecessary for frameshifting, and initiation at AUG codons within the stem-loop appeared to be inhibited. Like viruses that infect hosts in other kingdoms, plant viruses also can induce frameshifting in translation of their genes.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon Frameshift Mutation Glucuronidase/analysis,genetics Molecular Sequence Data Oligodeoxyribonucleotides Open Reading Frames Plant Viruses/genetics,isolation & purification Plants/genetics,microbiology Plasmids Polymerase Chain Reaction Protein Biosynthesis Protoplasts/physiology
Chemicals
Codon Oligodeoxyribonucleotides Glucuronidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brault V
Plant Pathology Department, Iowa State University, Ames 50011.
Miller W A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.6.2262
Published
1992-03-15
Pages
2262-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48637
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.6.2262 PMC Full Text View journal details
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