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PMID: 2053281 Published · ppublish English Journal Article

Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines.

Virology ·Vol. 183 ·No. 1 ·1991-07-00 ·Pages 215-24

Moosmayer D, Reil H, Ausmeier M, Scharf JG, Hauser H, Jentsch KD, Hunsmann G

Abstract

Expression, ribosomal frameshifting, and proteolytic processing of HIV-1 GAG and POL proteins were investigated in heterologous mammalian cells in order to elucidate the influence of the cellular background on these events. DNA fragments encoded by the gag and pol region were expressed in two rodent cell lines, LTK- and BHK. Both stably transfected cell lines continuously produce recombinant proteins which react with HIV-specific antisera. The GAG precursor and a 39-kDa proteolytic fragment thereof were the major recombinant proteins detected. Expression of the gag-pol region leads to the production of the GAG-POL precursor. Ribosomal frameshifting at the HIV-1 shifty sequence to a typical extent could be positively demonstrated by an enzyme assay. Despite the presence of the viral protease within the GAG-POL precursors, proteolytic processing of the HIV-derived polyproteins was extremely inefficient. The efficiency could not be enhanced by overexpression of the HIV-1 protease encoding region.

MeSH Terms
Animals Base Sequence Cell Line Chlorocebus aethiops Cricetinae Frameshift Mutation Gene Expression Gene Products, gag/genetics,metabolism Gene Products, pol/genetics,metabolism HIV Protease/metabolism HIV-1/genetics Humans Hydrolysis Mice Molecular Sequence Data Protein Processing, Post-Translational Substrate Specificity Transfection
Chemicals
Gene Products, gag Gene Products, pol HIV Protease
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Moosmayer D
Deutsches Primatenzentrum, Abt. Virologie und Immunologie, Göttingen.
Reil H
Ausmeier M
Scharf J G
Hauser H
Jentsch K D
Hunsmann G
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(91)90134-w
Published
1991-07-00
Pages
215-24
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7131782
Subset
IM
External Links
PubMed source DOI: 10.1016/0042-6822(91)90134-w PMC Full Text View journal details
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