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PMID: 2464705 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression and processing of human immunodeficiency virus type 1 gag and pol genes by cells infected with a recombinant vaccinia virus.

Journal of virology ·Vol. 63 ·No. 3 ·1989-03-00 ·Pages 1451-4

Gowda SD, Stein BS, Steimer KS, Engleman EG

Abstract

Human cells infected with a recombinant vaccinia virus containing human immunodeficiency virus type 1 gag-pol genes produced large amounts of human immunodeficiency virus gag proteins beginning at 1 h and peaking at 48 h postinfection. We show that these polyproteins are processed accurately into mature forms and that the viral polymerase gene is encoded as a 160-kilodalton gag-pol fusion protein, most likely by translational frameshifting from the gag into the pol reading frame.

MeSH Terms
Cells, Cultured DNA, Recombinant Gene Products, gag HIV/genetics Molecular Weight Protein Processing, Post-Translational RNA-Directed DNA Polymerase/genetics Retroviridae Proteins/genetics Vaccinia virus
Chemicals
DNA, Recombinant Gene Products, gag Retroviridae Proteins RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gowda S D
Department of Pathology, Stanford University School of Medicine, California 94305.
Stein B S
Steimer K S
Engleman E G
References (19)
19 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2).
    Science. 1985 Feb 1;227(4686):484-92 PMID: 2578227
  4. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  5. Complete nucleotide sequence of the AIDS virus, HTLV-III.
    Nature. 1985 Jan 24-30;313(6000):277-84 PMID: 2578615
  6. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  7. Murine leukemia virus maturation: protease region required for conversion from "immature" to "mature" core form and for virus infectivity.
    Virology. 1985 Sep;145(2):280-92 PMID: 2411050
  8. Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV.
    Science. 1986 Mar 14;231(4743):1289-91 PMID: 2418504
  9. Bovine leukemia virus protease: purification, chemical analysis, and in vitro processing of gag precursor polyproteins.
    J Virol. 1986 Mar;57(3):826-32 PMID: 3005629
  10. HTLV-III gag protein is processed in yeast cells by the virus pol-protease.
    Science. 1986 Mar 28;231(4745):1580-4 PMID: 2420008
  11. Use of vaccinia virus as a neuropeptide expression vector.
    Methods Enzymol. 1986;124:295-309 PMID: 3012250
  12. Proteolytic processing of avian sarcoma and leukosis viruses pol-endo recombinant proteins reveals another pol gene domain.
    J Virol. 1987 Feb;61(2):534-42 PMID: 2433465
  13. pH-independent HIV entry into CD4-positive T cells via virus envelope fusion to the plasma membrane.
    Cell. 1987 Jun 5;49(5):659-68 PMID: 3107838
  14. HIV-specific cytotoxic T lymphocytes in seropositive individuals.
    Nature. 1987 Jul 23-29;328(6128):345-8 PMID: 3496541
  15. Characterization of ribosomal frameshifting in HIV-1 gag-pol expression.
    Nature. 1988 Jan 21;331(6153):280-3 PMID: 2447506
  16. Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8903-6 PMID: 3321060
  17. Biochemical and immunological analysis of human immunodeficiency virus gag gene products p17 and p24.
    J Virol. 1988 Mar;62(3):795-801 PMID: 3123712
  18. Processing of gag precursor polyprotein of human T-cell leukemia virus type I by virus-encoded protease.
    J Virol. 1988 Oct;62(10):3718-28 PMID: 2843670
  19. Cleavage of Rous sarcoma viral polypeptide precursor into internal structural proteins in vitro involves viral protein p15.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):911-5 PMID: 191840
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.3.1451-1454.1989
Published
1989-03-00
Pages
1451-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247850
Subset
IM
Grants
NIAID NIH HHS · AI25922 · United States
NIAID NIH HHS · AI72657 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.3.1451-1454.1989 PMC Full Text View journal details
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