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PMID: 1542692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 5 ·1992-03-01 ·Pages 1949-53

White RJ, Jackson SP, Rigby PW

Abstract

The major class of vertebrate genes transcribed by RNA polymerase (EC 2.7.7.6) III, which includes 5S rRNA genes, tRNA genes, and the adenovirus VA genes, is characterized by split internal promoters and no absolute dependence upon specific upstream sequences. Fractionation experiments have shown that transcription of such genes requires two general RNA polymerase III-specific factors, TFIIIB and TFIIIC. We now demonstrate that a third general factor is also employed by these genes. This is the TATA-box-binding protein originally identified as being a component of the general RNA polymerase II transcription factor TFIID. This protein is involved in the transcription by RNA polymerase III of every template tested, even though the promoters of VA and most vertebrate tRNA and 5S rRNA genes do not contain recognizable TATA elements.

MeSH Terms
Base Sequence Binding, Competitive DNA-Binding Proteins/physiology HeLa Cells Humans In Vitro Techniques Macromolecular Substances Molecular Sequence Data Oligodeoxyribonucleotides/chemistry RNA Polymerase III/metabolism Recombinant Proteins/metabolism Regulatory Sequences, Nucleic Acid TATA Box TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors/chemistry,physiology Transcription, Genetic
Chemicals
DNA-Binding Proteins Macromolecular Substances Oligodeoxyribonucleotides Recombinant Proteins TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors RNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
White R J
Wellcome/CRC Institute, Cambridge, United Kingdom.
Jackson S P
Rigby P W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.5.1949
Published
1992-03-01
Pages
1949-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48571
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1073/pnas.89.5.1949 PMC Full Text View journal details
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