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PMID: 2050122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.

The EMBO journal ·Vol. 10 ·No. 7 ·1991-07-00 ·Pages 1853-62

Simmen KA, Bernués J, Parry HD, Stunnenberg HG, Berkenstam A, Cavallini B, Egly JM, Mattaj IW

Abstract

We present evidence that transcription factor TFIID, known for its central role in transcription by RNA polymerase II, is also involved in RNA polymerase III transcription of the human U6 snRNA gene. Recombinant human TFIID, expressed either via a vaccinia virus vector in HeLa cells or in Escherichia coli, affects U6 transcription in three different in vitro assays. First, TFIID-containing fractions stimulate U6 transcription in reactions containing rate-limiting amounts of HeLa nuclear extract. Second, TFIID addition relieves transcriptional exclusion between two competing U6 templates. Third, TFIID can replace one of two heat labile fractions essential for U6 transcription. Thus, at least one basal transcription factor is involved in transcription by two different RNA polymerases.

Related Genes
U6
MeSH Terms
Base Sequence Gene Expression Regulation HeLa Cells Hot Temperature Humans Promoter Regions, Genetic RNA Polymerase III/genetics,physiology RNA, Small Nuclear/biosynthesis,genetics Recombinant Proteins/biosynthesis,genetics TATA Box Templates, Genetic Transcription Factor TFIID Transcription Factors/genetics Transcription, Genetic
Chemicals
RNA, Small Nuclear Recombinant Proteins Transcription Factor TFIID Transcription Factors RNA Polymerase III
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Simmen K A
European Molecular Biology Laboratory, Heidelberg, FRG.
Bernués J
Parry H D
Stunnenberg H G
Berkenstam A
Cavallini B
Egly J M
Mattaj I W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-07-00
Pages
1853-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452860
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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