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PMID: 3262058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two different liver-specific factors stimulate in vitro transcription from the human alpha 1-antitrypsin promoter.

The EMBO journal ·Vol. 7 ·No. 7 ·1988-07-00 ·Pages 2075-87

Monaci P, Nicosia A, Cortese R

Abstract

The region from -137 to -2 of the human alpha 1-antitrypsin (alpha 1AT) promoter directs liver-specific in vitro transcription. Two cis-acting elements, A and B, have been identified within this segment by site-directed mutagenesis. Competition with synthetic oligonucleotides corresponding either to the A or to the B sequence inhibits transcription from the wild-type promoter in vitro. Cis-linked A and B elements mediate liver-specific transcription from a truncated HSV-TK promoter in vitro. Five different proteins, LF-A1, LF-A2, LF-B1, LF-B2 and LF-C, bind to the alpha 1AT promoter in liver extracts. LF-A1 and LF-B1 are positive transcriptional factors which bind to the A and B elements respectively. Their absence in spleen provides an explanation for the liver specificity of transcription. A protein similar to LF-B2 is present in spleen. Binding of LF-B1 and LF-B2 to the alpha 1AT promoter is mutually exclusive, suggesting that LF-B2 might be a repressor.

MeSH Terms
Base Sequence Cell Nucleus/metabolism Deoxyribonuclease I Genes Humans Liver/metabolism Molecular Sequence Data Nuclear Proteins/metabolism Plasmids Promoter Regions, Genetic Spleen/metabolism Transcription Factors/metabolism Transcription, Genetic alpha 1-Antitrypsin/genetics
Chemicals
Nuclear Proteins Transcription Factors alpha 1-Antitrypsin Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Monaci P
European Molecular Biology Laboratory, Heidelberg, FRG.
Nicosia A
Cortese R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-07-00
Pages
2075-87
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454491
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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