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PMID: 226978 Published · ppublish English Journal Article

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3755-9

Colbere-Garapin F, Chousterman S, Horodniceanu F, Kourilsky P, Garapin AC

Abstract

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.

MeSH Terms
Antigen-Antibody Reactions Cell Transformation, Viral DNA Restriction Enzymes/metabolism DNA, Recombinant Escherichia coli/genetics Genes Plasmids Simplexvirus/enzymology,genetics Thymidine Kinase/genetics,immunology
Chemicals
DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Colbere-Garapin F
Chousterman S
Horodniceanu F
Kourilsky P
Garapin A C
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3755
Published
1979-08-00
Pages
3755-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383912
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3755 PMC Full Text View journal details
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