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PMID: 1505519 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histone H3 N-terminal mutations allow hyperactivation of the yeast GAL1 gene in vivo.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3297-306

Mann RK, Grunstein M

Abstract

Recent work has shown that the yeast histone H4 N-terminus, while not essential for viability, is required for repression of the silent mating loci and activation of GAL1 and PHO5 promoters. Because histone H3 shares many structural features with histone H4 and is intimately associated with H4 in the assembled nucleosome, we asked whether H3 has similar functions. While the basic N-terminal domain of H3 is found to be non-essential (deletion of residues 4-40 of this 135 amino acid protein allows viability), its removal has only a minor effect on mating. Surprisingly, both deletions (of residues 4-15) and acetylation site substitutions (at residues 9, 14 and 18) within the N-terminus of H3 allow hyperactivation of the GAL1 promoter as well as a number of other GAL4-regulated genes including GAL2, GAL7 and GAL10. To a limited extent glucose repression is also alleviated by H3 N-terminal deletions. Expression of another inducible promoter, PHO5, is shown to be relatively unaffected. We conclude that the H3 and H4 N-termini have different functions in both the repression of the silent mating loci and in the regulation of GAL1.

Related Genes
GAL1 lacZ
MeSH Terms
Amino Acid Sequence Cell Division/genetics DNA Mutational Analysis DNA-Binding Proteins Enzyme Induction/drug effects Fungal Proteins/genetics,metabolism Galactose/pharmacology Gene Expression Regulation, Fungal Genes, Fungal Glucose/pharmacology Histones/genetics Molecular Sequence Data Mutagenesis Nucleosomes/metabolism Promoter Regions, Genetic/genetics RNA, Messenger/genetics Recombinant Fusion Proteins/genetics Repressor Proteins/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors beta-Galactosidase/genetics
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Histones Nucleosomes RNA, Messenger Recombinant Fusion Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors beta-Galactosidase Glucose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mann R K
Molecular Biology Institute, University of California, Los Angeles 90024.
Grunstein M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-09-00
Pages
3297-306
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556864
Subset
IM
Grants
NIGMS NIH HHS · GM23674 · United States
NIGMS NIH HHS · GM42421 · United States
External Links
PubMed source PMC Full Text View journal details
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