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PMID: 6390181 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 4 ·No. 10 ·1984-10-00 ·Pages 1985-98

Yocum RR, Hanley S, West R, Ptashne M

Abstract

We present the DNA sequence of a 914-base pair fragment from Saccharomyces cerevisiae that contains the GAL1-GAL10 divergent promoter, 140 base pairs of GAL10 coding sequence, and 87 base pairs of GAL1 coding sequence. From this fragment, we constructed four pairs of GAL1-lacZ and GAL10-lacZ fusions on various types of yeast plasmid vectors. On each type of vector, the fused genes were induced by galactose and repressed by glucose. The response of a GAL1-lacZ fusion to gal4 and gal80 regulatory mutations was similar to the response of intact chromosomal GAL1 and GAL10 genes. A set of deletions that removed various portions of the GAL10 regulatory sequences from a GAL10-CYC1-lacZ fusion was constructed in vitro. These deletions defined a relatively guanine-cytosine-rich region of 45 base pairs that contained sequences necessary for full-strength galactose induction and an adjacent guanine-cytosine rich 55 base pairs that contained sequences sufficient for weak induction.

MeSH Terms
Alleles Base Sequence Chromosome Deletion DNA/analysis Gene Expression Regulation Models, Genetic Operon Phenotype Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yocum R R
Hanley S
West R
Ptashne M
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53 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.10.1985-1998.1984
Published
1984-10-00
Pages
1985-98
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369015
Subset
IM
Grants
NIAID NIH HHS · AI18589 · United States
NIGMS NIH HHS · GM32308 · United States
Databases
GENBANK
K02115
External Links
PubMed source DOI: 10.1128/mcb.4.10.1985-1998.1984 PMC Full Text View journal details
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