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PMID: 14691136 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 163 ·No. 6 ·2003-12-22 ·Pages 1255-66

Estrada P, Kim J, Coleman J, Walker L, Dunn B, Takizawa P, Novick P, Ferro-Novick S

Abstract

Myo4p is a nonessential type V myosin required for the bud tip localization of ASH1 and IST2 mRNA. These mRNAs associate with Myo4p via the She2p and She3p proteins. She3p is an adaptor protein that links Myo4p to its cargo. She2p binds to ASH1 and IST2 mRNA, while She3p binds to both She2p and Myo4p. Here we show that Myo4p and She3p, but not She2p, are required for the inheritance of cortical ER in the budding yeast Saccharomyces cerevisiae. Consistent with this observation, we find that cortical ER inheritance is independent of mRNA transport. Cortical ER is a dynamic network that forms cytoplasmic tubular connections to the nuclear envelope. ER tubules failed to grow when actin polymerization was blocked with the drug latrunculin A (Lat-A). Additionally, a reduction in the number of cytoplasmic ER tubules was observed in Lat-A-treated and myo4Delta cells. Our results suggest that Myo4p and She3p facilitate the growth and orientation of ER tubules.

MeSH Terms
Actin Cytoskeleton/drug effects,metabolism Bridged Bicyclo Compounds, Heterocyclic/pharmacology Cell Division/physiology Cytoplasmic Streaming/physiology Endoplasmic Reticulum/metabolism,ultrastructure Intracellular Membranes/metabolism,ultrastructure Mutation/genetics Myosin Heavy Chains/genetics,metabolism Myosin Type V/genetics,metabolism RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism Saccharomyces cerevisiae/metabolism,ultrastructure Saccharomyces cerevisiae Proteins/genetics,metabolism Thiazoles/pharmacology Thiazolidines
Chemicals
Bridged Bicyclo Compounds, Heterocyclic Myo4 protein, S cerevisiae RNA, Messenger RNA-Binding Proteins SHE3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Thiazoles Thiazolidines Myosin Type V Myosin Heavy Chains latrunculin A
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Estrada Paula
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA.
Kim Jiwon
Coleman Jeff
Walker Lee
Dunn Brian
Takizawa Peter
Novick Peter
Ferro-Novick Susan
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200304030
Published
2003-12-22
Pages
1255-66
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173705
Subset
IM
Corrections
CommentIn
PMID: 14691131
External Links
PubMed source DOI: 10.1083/jcb.200304030 PMC Full Text View journal details
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