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PMID: 10792032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 10 ·2000-05-09 ·Pages 5273-8

Takizawa PA, Vale RD

Abstract

In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of the five She proteins, She2p, She3p, and Myo4p, colocalize with Ash1 mRNA in vivo and coimmunoprecipitate with Ash1 mRNA from cell extracts. We also find that She3p binds to Myo4p in the absence of RNA and She2p is required for binding She3p-Myo4p to Ash1 mRNA. These results suggest that She3p acts as an adapter protein that docks the myosin motor onto an Ash1-She2p ribonucleoprotein complex.

MeSH Terms
Binding Sites Carrier Proteins/isolation & purification,metabolism DNA-Binding Proteins Fungal Proteins/isolation & purification,metabolism Models, Biological Myosin Heavy Chains Myosin Type V Myosins/isolation & purification,metabolism RNA, Fungal/genetics,isolation & purification,metabolism RNA, Messenger/genetics,isolation & purification,metabolism Repressor Proteins Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics,isolation & purification Zinc Fingers
Chemicals
ASH1 protein, S cerevisiae Carrier Proteins DNA-Binding Proteins Fungal Proteins Myo4 protein, S cerevisiae RNA, Fungal RNA, Messenger Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Myosin Type V Myosin Heavy Chains Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takizawa P A
Department of Cellular and Molecular Pharmacology; and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-0450, USA.
Vale R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.080585897
Published
2000-05-09
Pages
5273-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC25818
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038499 · United States
PHS HHS · 38496 · United States
External Links
PubMed source DOI: 10.1073/pnas.080585897 PMC Full Text View journal details
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