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PMID: 1986005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of components involved in protein transport and processing through the yeast Golgi apparatus.

The Journal of cell biology ·Vol. 112 ·No. 1 ·1991-01-00 ·Pages 27-37

Franzusoff A, Redding K, Crosby J, Fuller RS, Schekman R

Abstract

Saccharomyces cerevisiae sec7 mutants exhibit pleiotropic deficiencies in the transit of proteins through the Golgi apparatus, and elaborate an array of Golgi apparatus-like cisternae at a restrictive growth temperature (37 degrees C). The SEC7 gene encodes an essential high-molecular weight protein (227 kD) that is phosphorylated in vivo. In cell lysates, Sec7 protein (Sec7p) is recovered in both sedimentable and soluble fractions. A punctate immunofluorescent pattern of Sec7p-associated structures seen in SEC cells coalesces in sec14 mutant yeast that accumulate exaggerated Golgi cisternae at 37 degrees C. Sec7p may function as a peripheral membrane protein that cycles between a soluble, cytosolic pool and a sedimentable, membrane-associated complex for its essential role in vesicular traffic through the Golgi apparatus. The transmembrane Kex2 protease, which processes precursors of secreted peptides within the yeast secretory pathway, is also localized by indirect immunofluorescence to multiple structures in the yeast cell (Redding, K., and R. Fuller, manuscript submitted for publication). In double-immunofluorescence labeling experiments, significant colocalization of Sec7 and Kex2 proteins was found. Colocalization of the two antigens, one implicated in protein transport through the Golgi apparatus and the other in processing within a late Golgi compartment, supports the conclusion that we have visualized the yeast Golgi apparatus.

MeSH Terms
Biological Transport/physiology Fungal Proteins/metabolism Genes, Fungal/physiology Golgi Apparatus/chemistry,enzymology Guanine Nucleotide Exchange Factors Proprotein Convertases Protein Processing, Post-Translational Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Serine Endopeptidases/analysis Subcellular Fractions/chemistry Subtilisins
Chemicals
Fungal Proteins Guanine Nucleotide Exchange Factors Saccharomyces cerevisiae Proteins Sec7 guanine nucleotide exchange factors Proprotein Convertases Serine Endopeptidases Subtilisins KEX2 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Franzusoff A
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
Redding K
Crosby J
Fuller R S
Schekman R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.112.1.27
Published
1991-01-00
Pages
27-37
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288806
Subset
IM
Grants
NIGMS NIH HHS · GM-36881 · United States
NIGMS NIH HHS · GM-39697 · United States
External Links
PubMed source DOI: 10.1083/jcb.112.1.27 PMC Full Text View journal details
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