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PMID: 3186695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 7852-6

Block MR, Glick BS, Wilcox CA, Wieland FT, Rothman JE

Abstract

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Antibodies, Monoclonal Biological Transport, Active/drug effects Carrier Proteins/isolation & purification,pharmacology Cell Line Cricetinae Cytosol/analysis Golgi Apparatus/metabolism
Chemicals
Antibodies, Monoclonal Carrier Proteins Adenosine Triphosphate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Block M R
Department of Biochemistry, Stanford University, CA 94305.
Glick B S
Wilcox C A
Wieland F T
Rothman J E
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.7852
Published
1988-11-00
Pages
7852-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282295
Subset
IM
Grants
NIDDK NIH HHS · DK 27044 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.21.7852 PMC Full Text View journal details
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