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PMID: 6253996 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transport of vesicular stomatitis virus glycoprotein in a cell-free extract.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 3870-4

Fries E, Rothman JE

Abstract

We describe a cell-free system in which the membrane glycoprotein of vesicular stomatitis virus is rapidly and efficiently transported to membranes of the Golgi complex by a process resembling intracellular protein transport. Transport in vitro is energy-dependent and is accompanied by terminal glycosylation of the membrane glycoprotein (dependent upon UDP-GlcNAc and resulting in resistance to endo-beta-N-acetylglucosaminidase H).

MeSH Terms
Animals Biological Transport, Active Cell Line Cell-Free System Cricetinae Female Glycoproteins/metabolism Golgi Apparatus/metabolism Membrane Proteins/metabolism Oligosaccharides/metabolism Ovary Vesicular stomatitis Indiana virus/metabolism Viral Proteins/metabolism
Chemicals
Glycoproteins Membrane Proteins Oligosaccharides Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fries E
Rothman J E
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.3870
Published
1980-07-00
Pages
3870-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349728
Subset
IM
Grants
NIGMS NIH HHS · GM 25662 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.7.3870 PMC Full Text View journal details
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