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PMID: 11553703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Aux1p/Swa2p is required for cortical endoplasmic reticulum inheritance in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 12 ·No. 9 ·2001-09-00 ·Pages 2614-28

Du Y, Pypaert M, Novick P, Ferro-Novick S

Abstract

In the yeast Saccharomyces cerevisiae, the endoplasmic reticulum (ER) is found at the periphery of the cell and around the nucleus. The segregation of ER through the mother-bud neck may occur by more than one mechanism because perinuclear, but not peripheral ER, requires microtubules for this event. To identify genes whose products are required for cortical ER inheritance, we have used a Tn3-based transposon library to mutagenize cells expressing a green fluorescent protein-tagged ER marker protein (Hmg1p). This approach has revealed that AUX1/SWA2 plays a role in ER inheritance. The COOH terminus of Aux1p/Swa2p contains a J-domain that is highly related to the J-domain of auxilin, which stimulates the uncoating of clathrin-coated vesicles. Deletion of the J-domain of Aux1p/Swa2p leads to vacuole fragmentation and membrane accumulation but does not affect the migration of peripheral ER into daughter cells. These findings suggest that Aux1p/Swa2p may be a bifunctional protein with roles in membrane traffic and cortical ER inheritance. In support of this hypothesis, we find that Aux1p/Swa2p localizes to ER membranes.

MeSH Terms
ADP-Ribosylation Factor 1/genetics,metabolism Amino Acid Sequence Base Sequence Carrier Proteins/chemistry,genetics,metabolism Cell Division Cell Membrane/metabolism,ultrastructure Cloning, Molecular Endoplasmic Reticulum/metabolism,ultrastructure Extrachromosomal Inheritance Microscopy, Electron Microscopy, Fluorescence Molecular Sequence Data Mutagenesis, Site-Directed Mutation/genetics Phosphoproteins/chemistry,genetics,metabolism Protein Structure, Tertiary Saccharomyces cerevisiae/cytology,genetics,ultrastructure Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism Vacuoles/metabolism,ultrastructure Vesicular Transport Proteins
Chemicals
Carrier Proteins Phosphoproteins SWA2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vesicular Transport Proteins ADP-Ribosylation Factor 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Du Y
Department of Cell Biology, Yale University, New Haven, Connecticut 06519-1418, USA.
Pypaert M
Novick P
Ferro-Novick S
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.9.2614
Published
2001-09-00
Pages
2614-28
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC59699
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.12.9.2614 PMC Full Text View journal details
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