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PMID: 9199166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles.

The Journal of cell biology ·Vol. 137 ·No. 7 ·1997-06-30 ·Pages 1495-509

Walch-Solimena C, Collins RN, Novick PJ

Abstract

The small GTPase Sec4p is required for vesicular transport at the post-Golgi stage of yeast secretion. Here we present evidence that mutations in SEC2, itself an essential gene that acts at the same stage of the secretory pathway, cause Sec4p to mislocalize as a result of a random rather than a polarized accumulation of vesicles. Sec2p and Sec4p interact directly, with the nucleotide-free conformation of Sec4p being the preferred state for interaction with Sec2p. Sec2p functions as an exchange protein, catalyzing the dissociation of GDP from Sec4 and promoting the binding of GTP. We propose that Sec2p functions to couple the activation of Sec4p to the polarized delivery of vesicles to the site of exocytosis.

MeSH Terms
Amino Acid Sequence Biological Transport/genetics Cell Polarity Fungal Proteins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Gene Expression Regulation, Fungal Golgi Apparatus/metabolism,ultrastructure Guanine Nucleotide Exchange Factors Molecular Sequence Data Mutation Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins Sequence Alignment rab GTP-Binding Proteins
Chemicals
Fungal Proteins Guanine Nucleotide Exchange Factors SEC2 protein, S cerevisiae Saccharomyces cerevisiae Proteins GTP-Binding Proteins SEC4 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Walch-Solimena C
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Collins R N
Novick P J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.137.7.1495
Published
1997-06-30
Pages
1495-509
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2137815
Subset
IM
Grants
NIGMS NIH HHS · GM35370 · United States
NCI NIH HHS · CA46128 · United States
External Links
PubMed source DOI: 10.1083/jcb.137.7.1495 PMC Full Text View journal details
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