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PMID: 8157010 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway.

The EMBO journal ·Vol. 13 ·No. 7 ·1994-04-01 ·Pages 1718-28

Garrett MD, Zahner JE, Cheney CM, Novick PJ

Abstract

GTP binding proteins of the Sec4/Ypt/rab family regulate distinct vesicular traffic events in eukaryotic cells. We have cloned GDI1, an essential homolog of bovine rab GDI (GDP dissociation inhibitor) from the yeast Saccharomyces cerevisiae. Analogous to the bovine protein, purified Gdi1p slows the dissociation of GDP from Sec4p and releases the GDP-bound form from yeast membranes. Depletion of Gdi1p in vivo leads to loss of the soluble pool of Sec4p and inhibition of protein transport at multiple stages of the secretory pathway. Complementation analysis indicates that GDI1 is allelic to sec19-1. These results establish that Gdi1p plays an essential function in membrane traffic and are consistent with a role for Gdi1p in the recycling of proteins of the Sec4/Ypt/rab family from their target membranes back to their vesicular pools.

Related Genes
GDI1
MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Cloning, Molecular Endoplasmic Reticulum/physiology,ultrastructure Fungal Proteins/metabolism GTP-Binding Proteins/genetics,metabolism Genes, Fungal/genetics Glycoside Hydrolases/metabolism Golgi Apparatus/physiology,ultrastructure Guanine Nucleotide Dissociation Inhibitors Isoquinolines/metabolism Molecular Sequence Data Saccharomyces cerevisiae/genetics,growth & development,metabolism,ultrastructure Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid beta-Fructofuranosidase rab GTP-Binding Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors
Chemicals
Fungal Proteins GDP dissociation inhibitor 1 Guanine Nucleotide Dissociation Inhibitors Isoquinolines Saccharomyces cerevisiae Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors lucifer yellow Glycoside Hydrolases beta-Fructofuranosidase GTP-Binding Proteins SEC4 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garrett M D
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.
Zahner J E
Cheney C M
Novick P J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-04-01
Pages
1718-28
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395005
Subset
IM
Grants
NCI NIH HHS · CA46128 · United States
NIGMS NIH HHS · GM35370 · United States
Databases
GENBANK
S69371
External Links
PubMed source PMC Full Text View journal details
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