Home LiteratureArticle Details
PMID: 1901952 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mammalian inhibitory GDP/GTP exchange protein (GDP dissociation inhibitor) for smg p25A is active on the yeast SEC4 protein.

Molecular and cellular biology ·Vol. 11 ·No. 5 ·1991-05-00 ·Pages 2909-12

Sasaki T, Kaibuchi K, Kabcenell AK, Novick PJ, Takai Y

Abstract

Evidence is accumulating that smg p25A, a small GTP-binding protein, may be involved in the regulated secretory processes of mammalian cells. The SEC4 protein is known to be required for constitutive secretion in yeast cells. We show here that the mammalian GDP dissociation inhibitor (GDI), which was identified by its action on smg p25A, is active on the yeast SEC4 protein in inhibiting the GDP/GTP exchange reaction and is capable of forming a complex with the GDP-bound form of the SEC4 protein but not with the GTP-bound form. These results together with our previous findings that smg p25A GDI is found in mammalian cells with both regulated and constitutive secretion types suggest that smg p25A GDI plays a role in both regulated and constitutive secretory processes, although smg p25A itself may be involved only in regulated secretory processes. These results also suggest that a GDI for the SEC4 protein is present in yeast cells.

MeSH Terms
Animals Brain/metabolism Cattle Cell Membrane/metabolism Cytosol/metabolism Fungal Proteins/metabolism GTP-Binding Proteins/antagonists & inhibitors,isolation & purification,metabolism Guanine Nucleotide Dissociation Inhibitors Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Kinetics Macromolecular Substances Protein Binding Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins rab GTP-Binding Proteins rab3 GTP-Binding Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors
Chemicals
Fungal Proteins Guanine Nucleotide Dissociation Inhibitors Macromolecular Substances Saccharomyces cerevisiae Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors Guanosine Diphosphate Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate GTP-Binding Proteins SEC4 protein, S cerevisiae rab GTP-Binding Proteins rab3 GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sasaki T
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kaibuchi K
Kabcenell A K
Novick P J
Takai Y
References (24)
24 references, click to expand
  1. Tissue and subcellular distributions of an inhibitory GDP/GTP exchange protein (GDI) for smg p25A by use of its antibody.
    Biochem Biophys Res Commun. 1991 Jan 31;174(2):556-63 PMID: 1899568
  2. Molecular cloning and characterization of a novel type of regulatory protein (GDI) for smg p25A, a ras p21-like GTP-binding protein.
    Mol Cell Biol. 1990 Aug;10(8):4116-22 PMID: 2115118
  3. The ras-like protein p25rab3A is partially cytosolic and is expressed only in neural tissue.
    Mol Cell Biol. 1989 Nov;9(11):4807-11 PMID: 2513478
  4. Tissue distribution of smg p25A, a ras p21-like GTP-binding protein, studied by use of a specific monoclonal antibody.
    Biochem Biophys Res Commun. 1989 Aug 15;162(3):1438-45 PMID: 2504158
  5. Tissue-specific expression of a novel GTP-binding protein (smg p25A) mRNA and its increase by nerve growth factor and cyclic AMP in rat pheochromocytoma PC-12 cells.
    Biochem Biophys Res Commun. 1989 Jan 31;158(2):377-85 PMID: 2537071
  6. Intrasynaptosomal distribution of the ras, rho and smg-25A GTP-binding proteins in bovine brain.
    Brain Res Mol Brain Res. 1989 Nov;6(2-3):167-76 PMID: 2515409
  7. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  8. Purification and characterization of a novel GTP-binding protein with a molecular weight of 24,000 from bovine brain membranes.
    J Biol Chem. 1988 Feb 25;263(6):2897-904 PMID: 3125171
  9. Multiple small molecular weight GTP-binding proteins in bovine brain cytosol. Purification and characterization of a 24KDa protein.
    Biochem Biophys Res Commun. 1988 Sep 30;155(3):1284-92 PMID: 3140807
  10. Nucleotide and deduced amino acid sequences of a GTP-binding protein family with molecular weights of 25,000 from bovine brain.
    J Biol Chem. 1988 Aug 15;263(23):11071-4 PMID: 3136152
  11. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast.
    Cell. 1988 Jun 3;53(5):753-68 PMID: 3131018
  12. Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues.
    Oncogene. 1988 Aug;3(2):231-4 PMID: 2457862
  13. ras genes.
    Annu Rev Biochem. 1987;56:779-827 PMID: 3304147
  14. A ras-like protein is required for a post-Golgi event in yeast secretion.
    Cell. 1987 May 22;49(4):527-38 PMID: 3552249
  15. Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins.
    J Biol Chem. 1990 Sep 25;265(27):16626-34 PMID: 2118909
  16. Association of the GTP-binding protein Rab3A with bovine adrenal chromaffin granules.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5692-6 PMID: 2165599
  17. Regulation of reversible binding of smg p25A, a ras p21-like GTP-binding protein, to synaptic plasma membranes and vesicles by its specific regulatory protein, GDP dissociation inhibitor.
    J Biol Chem. 1990 Aug 5;265(22):13007-15 PMID: 2115887
  18. Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway.
    EMBO J. 1990 Aug;9(8):2375-83 PMID: 2114975
  19. Purification and characterization of a novel GTP-binding protein with a Mr value of 24,000 from rat liver.
    Biochem Biophys Res Commun. 1990 Jun 15;169(2):816-23 PMID: 2162674
  20. Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic.
    J Biol Chem. 1990 Jun 5;265(16):9366-72 PMID: 2111819
  21. Localization and subcellular distribution of smg p25A, a ras p21-like GTP-binding protein, in rat brain.
    J Biol Chem. 1990 Jul 15;265(20):11872-9 PMID: 2114404
  22. rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles.
    Proc Natl Acad Sci U S A. 1990 Mar;87(5):1988-92 PMID: 2155429
  23. Purification and characterization from bovine brain cytosol of a protein that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A, a ras p21-like GTP-binding protein.
    J Biol Chem. 1990 Feb 5;265(4):2333-7 PMID: 2105320
  24. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.
    J Biol Chem. 1989 Jul 25;264(21):12394-401 PMID: 2501306
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.5.2909-2912.1991
Published
1991-05-00
Pages
2909-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360085
Subset
IM
Grants
NIGMS NIH HHS · GM35370 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.5.2909-2912.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2