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PMID: 2155429 Published · ppublish English Journal Article

rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 5 ·1990-03-00 ·Pages 1988-92

Fischer von Mollard G, Mignery GA, Baumert M, Perin MS, Hanson TJ, Burger PM, Jahn R, Südhof TC

Abstract

rab3, a low molecular weight GTP-binding protein, is primarily expressed in brain, where it is present in soluble and membrane-bound forms. Membrane-bound rab3 in brain is exclusively localized on synaptic vesicles, the secretory organelles of the synapse that store and release neurotransmitters. rab3 is also expressed in endocrine tissues such as the adrenal medulla, where it is found together with other synaptic vesicle proteins on microvesicles distinct from chromaffin granules. The tight binding of rab3 to membranes correlates with hydrophobic modifications that are different in the membrane-bound and soluble forms of rab3. The results demonstrate the exclusive targeting of a small GTP-binding protein to secretory vesicles of a subset of the regulated pathway of secretion.

MeSH Terms
Amino Acid Sequence Animals Antibodies Cell Fractionation Cerebral Cortex/metabolism Chromaffin Granules/metabolism Electrophoresis, Polyacrylamide Gel GTP-Binding Proteins/isolation & purification,metabolism Molecular Sequence Data Molecular Weight Nerve Tissue Proteins/isolation & purification,metabolism Protein Binding Rats Synaptic Vesicles/metabolism rab3 GTP-Binding Proteins
Chemicals
Antibodies Nerve Tissue Proteins GTP-Binding Proteins rab3 GTP-Binding Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Fischer von Mollard G
Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, Federal Republic of Germany.
Mignery G A
Baumert M
Perin M S
Hanson T J
Burger P M
Jahn R
Südhof T C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.5.1988
Published
1990-03-00
Pages
1988-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53610
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.5.1988 PMC Full Text View journal details
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