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PMID: 8570643 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo examination of membrane protein localization and degradation with green fluorescent protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 2 ·1996-01-23 ·Pages 828-33

Hampton RY, Koning A, Wright R, Rine J

Abstract

To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

MeSH Terms
Base Sequence Cell Compartmentation Flow Cytometry Genes, Reporter Green Fluorescent Proteins Hydroxymethylglutaryl CoA Reductases/genetics,metabolism Luminescent Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Microscopy, Fluorescence Models, Molecular Molecular Sequence Data Recombinant Fusion Proteins/biosynthesis Saccharomyces cerevisiae/genetics,metabolism,ultrastructure
Chemicals
Luminescent Proteins Membrane Proteins Recombinant Fusion Proteins Green Fluorescent Proteins Hydroxymethylglutaryl CoA Reductases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hampton R Y
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Koning A
Wright R
Rine J
References (11)
11 references, click to expand
  1. Structure of the yeast endoplasmic reticulum: localization of ER proteins using immunofluorescence and immunoelectron microscopy.
    Yeast. 1991 Dec;7(9):891-911 PMID: 1803815
  2. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.
    Biochemistry. 1993 Feb 9;32(5):1212-8 PMID: 8448132
  3. DiOC6 staining reveals organelle structure and dynamics in living yeast cells.
    Cell Motil Cytoskeleton. 1993;25(2):111-28 PMID: 7686821
  4. Green fluorescent protein as a marker for gene expression.
    Science. 1994 Feb 11;263(5148):802-5 PMID: 8303295
  5. Regulated degradation of HMG-CoA reductase, an integral membrane protein of the endoplasmic reticulum, in yeast.
    J Cell Biol. 1994 Apr;125(2):299-312 PMID: 8163547
  6. The regulated degradation of 3-hydroxy-3-methylglutaryl-CoA reductase requires a short-lived protein and occurs in the endoplasmic reticulum.
    J Biol Chem. 1990 Dec 15;265(35):22004-10 PMID: 2254343
  7. Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus.
    J Cell Biol. 1988 Jul;107(1):101-14 PMID: 3292536
  8. Fluorescent labeling of endoplasmic reticulum.
    Methods Cell Biol. 1989;29:125-35 PMID: 2643757
  9. KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene.
    Cell. 1989 Jun 30;57(7):1211-21 PMID: 2661018
  10. Transmission electron microscopy and immunocytochemical studies of yeast: analysis of HMG-CoA reductase overproduction by electron microscopy.
    Methods Cell Biol. 1989;31:473-512 PMID: 2674630
  11. Alterations in the rates of synthesis and degradation of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase produced by cholestyramine and mevinolin.
    J Biol Chem. 1983 Sep 10;258(17):10219-22 PMID: 6554277
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.2.828
Published
1996-01-23
Pages
828-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40142
Subset
IM
Grants
NIGMS NIH HHS · GM35827 · United States
NIGMS NIH HHS · GM45726 · United States
NIGMS NIH HHS · T32GM07270 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.2.828 PMC Full Text View journal details
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