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PMID: 11285273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 153 ·No. 1 ·2001-04-02 ·Pages 47-62

Rossanese OW, Reinke CA, Bevis BJ, Hammond AT, Sears IB, O'Connor J, Glick BS

Abstract

In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells.

MeSH Terms
Actins/physiology Biomarkers Bridged Bicyclo Compounds, Heterocyclic/pharmacology Carrier Proteins/genetics,metabolism,physiology Cell Cycle Proteins/genetics,metabolism,physiology Drug Resistance, Microbial Fungal Proteins/genetics,metabolism Golgi Apparatus/physiology Green Fluorescent Proteins Guanine Nucleotide Exchange Factors Luminescent Proteins/genetics,metabolism Membrane Proteins Mutagenesis Myosin Heavy Chains Myosin Type II Myosin Type V Myosins/genetics,metabolism,physiology Phenotype Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/drug effects,growth & development,metabolism,physiology Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Thiazoles/pharmacology Thiazolidines Transcription Factors/genetics,metabolism
Chemicals
Actins Biomarkers Bridged Bicyclo Compounds, Heterocyclic CDC1 protein, S cerevisiae Carrier Proteins Cell Cycle Proteins Fungal Proteins Guanine Nucleotide Exchange Factors Luminescent Proteins MYO2 protein, S cerevisiae MYO2 protein, S pombe Membrane Proteins RIC1 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Sec7 guanine nucleotide exchange factors Thiazoles Thiazolidines Transcription Factors Green Fluorescent Proteins Myosin Type II Myosin Type V Myosin Heavy Chains Myosins latrunculin A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rossanese O W
Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA.
Reinke C A
Bevis B J
Hammond A T
Sears I B
O'Connor J
Glick B S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.153.1.47
Published
2001-04-02
Pages
47-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2185536
Subset
IM
Grants
PHS HHS · 5-20942 · United States
NIGMS NIH HHS · GM61156 · United States
External Links
PubMed source DOI: 10.1083/jcb.153.1.47 PMC Full Text View journal details
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