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PMID: 1464330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

E.coli MukB protein involved in chromosome partition forms a homodimer with a rod-and-hinge structure having DNA binding and ATP/GTP binding activities.

The EMBO journal ·Vol. 11 ·No. 13 ·1992-12-00 ·Pages 5101-9

Niki H, Imamura R, Kitaoka M, Yamanaka K, Ogura T, Hiraga S

Abstract

mukB mutants of Escherichia coli are defective in the correct partitioning of replicated chromosomes. This results in the appearance of normal-sized anucleate (chromosome-less) cells during cell proliferation. Based on the nucleotide sequence of the mukB gene, the MukB protein of 177 kDa was predicted to be a filamentous protein with globular domains at the ends, and also having DNA binding and nucleotide binding abilities. Here we present evidence that the purified MukB protein possesses these characteristics. MukB forms a homodimer with a rod-and-hinge structure having a pair of large, C-terminal globular domains at one end and a pair of small, N-terminal globular domains at the opposite end; it tends to bend at a middle hinge site of the rod section. Chromatography in a DNA-cellulose column and the gel retardation assay revealed that MukB possesses DNA binding activity. Photoaffinity cross-linking experiments showed that MukB binds to ATP and GTP in the presence of Zn2+. Throughout the purification steps, acyl carrier protein was co-purified with MukB.

Related Genes
mukB
MeSH Terms
Acyl Carrier Protein/isolation & purification Adenosine Triphosphate/metabolism Amino Acid Sequence Animals Bacterial Proteins/chemistry,isolation & purification,metabolism,ultrastructure Blotting, Western Chromatography, Gel Chromosomal Proteins, Non-Histone Chromosomes, Bacterial Cross-Linking Reagents DNA-Binding Proteins/chemistry,isolation & purification,metabolism,ultrastructure Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Escherichia coli Proteins Guanosine Triphosphate/metabolism Microscopy, Electron Molecular Sequence Data Protein Conformation
Chemicals
Acyl Carrier Protein Bacterial Proteins Chromosomal Proteins, Non-Histone Cross-Linking Reagents DNA-Binding Proteins Escherichia coli Proteins MukB protein, E coli Guanosine Triphosphate Adenosine Triphosphate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Niki H
Department of Molecular Cell Biology, Kumamoto University School of Medicine, Japan.
Imamura R
Kitaoka M
Yamanaka K
Ogura T
Hiraga S
References (44)
44 references, click to expand
  1. Localization of acyl carrier protein in Escherichia coli.
    J Bacteriol. 1985 Apr;162(1):5-8 PMID: 3884600
  2. Chromosome partition in Escherichia coli requires postreplication protein synthesis.
    J Bacteriol. 1989 Oct;171(10):5405-9 PMID: 2676975
  3. Rotary shadowing of extended molecules dried from glycerol.
    J Ultrastruct Res. 1980 May;71(2):95-102 PMID: 6155474
  4. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition.
    Cell. 1983 Feb;32(2):351-60 PMID: 6297791
  5. Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli.
    J Bacteriol. 1983 Feb;153(2):693-9 PMID: 6337123
  6. Binding of the origin of replication of Escherichia coli to the outer membrane.
    Cell. 1982 Oct;30(3):915-23 PMID: 6754093
  7. Re-evaluation of the solution structure of acyl carrier protein.
    J Biol Chem. 1979 Oct 10;254(19):9778-85 PMID: 39930
  8. Chromosome and plasmid partition in Escherichia coli.
    Annu Rev Biochem. 1992;61:283-306 PMID: 1497312
  9. Colon cancer. Consider the coiled coil....
    Nature. 1991 May 16;351(6323):188-90 PMID: 1645848
  10. Mutants defective in chromosome partitioning in E. coli.
    Res Microbiol. 1991 Feb-Apr;142(2-3):189-94 PMID: 1925018
  11. TolC, an Escherichia coli outer membrane protein required for hemolysin secretion.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4776-80 PMID: 2112747
  12. The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli.
    EMBO J. 1991 Jan;10(1):183-93 PMID: 1989883
  13. Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation.
    Nature. 1991 Jun 27;351(6329):759-61 PMID: 2062368
  14. Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins.
    Nature. 1990 Sep 20;347(6290):256-61 PMID: 2144893
  15. Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allows ATP binding to its amino-terminal region.
    J Biol Chem. 1990 May 25;265(15):8760-5 PMID: 2160468
  16. Nucleotide sequence of the tolC gene of Escherichia coli.
    Nucleic Acids Res. 1990 Sep 25;18(18):5547 PMID: 2216730
  17. Positioning of replicated chromosomes in Escherichia coli.
    J Bacteriol. 1990 Jan;172(1):31-9 PMID: 2152907
  18. New topoisomerase essential for chromosome segregation in E. coli.
    Cell. 1990 Oct 19;63(2):393-404 PMID: 2170028
  19. Identification of dynamin, a novel mechanochemical enzyme that mediates interactions between microtubules.
    Cell. 1989 Nov 3;59(3):421-32 PMID: 2529977
  20. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells.
    J Bacteriol. 1989 Mar;171(3):1496-505 PMID: 2646284
  21. The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated.
    Cell. 1988 Jul 1;54(1):127-35 PMID: 2838178
  22. Structure and function of the F plasmid genes essential for partitioning.
    J Mol Biol. 1986 Nov 5;192(1):1-15 PMID: 3029390
  23. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  24. ParD: a new gene coding for a protein required for chromosome partitioning and septum localization in Escherichia coli.
    Mol Microbiol. 1987 Jul;1(1):73-81 PMID: 3330757
  25. Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants.
    J Bacteriol. 1987 Apr;169(4):1469-73 PMID: 3549687
  26. Partition of unit-copy miniplasmids to daughter cells. III. The DNA sequence and functional organization of the P1 partition region.
    J Mol Biol. 1985 Sep 20;185(2):261-72 PMID: 3903163
  27. The complete amino acid sequence of the acyl carrier protein of Escherichia coli.
    J Biol Chem. 1968 Dec 25;243(24):6420-31 PMID: 4882207
  28. Structural and functional analysis of the par region of the pSC 10 1 plasmid.
    Cell. 1984 Aug;38(1):191-201 PMID: 6088064
  29. Confirmation of molecular weight of Aspergillus oryzae alpha-amylase using the low angle laser light scattering technique in combination with high pressure silica gel chromatography.
    J Biochem. 1981 Feb;89(2):363-8 PMID: 6165712
  30. Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance.
    Cell. 1980 Jun;20(2):529-42 PMID: 6248248
  31. Bacterial chromosome segregation: evidence for DNA gyrase involvement in decatenation.
    Cell. 1984 Apr;36(4):1081-8 PMID: 6323018
  32. Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully.
    J Bacteriol. 1984 May;158(2):742-5 PMID: 6373730
  33. Assessment study on the use of the low angle laser light scattering technique for the estimation of molecular weight of the polypeptide forming a complex with sodium dodecyl sulfate.
    Biochim Biophys Acta. 1980 Nov 20;626(1):5-14 PMID: 7193052
  34. Dynamin is a GTPase stimulated to high levels of activity by microtubules.
    Nature. 1992 Feb 20;355(6362):733-5 PMID: 1311055
  35. Chromosome partitioning in Escherichia coli in the absence of dam-directed methylation.
    J Bacteriol. 1992 Apr;174(7):2388-90 PMID: 1551854
  36. Anion-induced conformational change of apo-electron-transferring flavoprotein.
    J Biochem. 1992 Mar;111(3):359-65 PMID: 1587798
  37. Experiments on chromosome separation and positioning in Escherichia coli.
    New Biol. 1991 May;3(5):475-86 PMID: 1883813
  38. The parD- mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA.
    Mol Microbiol. 1987 Nov;1(3):259-73 PMID: 2834621
  39. Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli.
    J Bacteriol. 1988 Sep;170(9):3967-77 PMID: 2842295
  40. Partition site of the P1 plasmid.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8544-7 PMID: 3317415
  41. Early replicative intermediates of Escherichia coli chromosome isolated from a membrane complex.
    EMBO J. 1986 Apr;5(4):787-91 PMID: 3519212
  42. Reconstitution of influenza virus RNA-nucleoprotein complexes structurally resembling native viral ribonucleoprotein cores.
    J Biol Chem. 1990 Jul 5;265(19):11151-5 PMID: 2358455
  43. Escherichia coli parA is an allele of the gyrB gene.
    Mol Gen Genet. 1989 May;217(1):178-81 PMID: 2549366
  44. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-12-00
Pages
5101-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556988
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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