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PMID: 2152907 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Positioning of replicated chromosomes in Escherichia coli.

Journal of bacteriology ·Vol. 172 ·No. 1 ·1990-01-00 ·Pages 31-9

Hiraga S, Ogura T, Niki H, Ichinose C, Mori H

Abstract

The positioning of replicated chromosomes at one-fourth and three-fourths of the cell length was inhibited when protein synthesis was inhibited by chloramphenicol or rifampin or by starvation for amino acids. Under these conditions, the progress of chromosome replication continued and replicated chromosomes were located close to each other as one nucleoid mass at midcell. Cells which already had two separate daughter chromosomes located at the cell quarters divided into two daughter cells under these conditions. When protein synthesis resumed, daughter chromosomes moved from midcell to the cell quarters, respectively, before any detectable increase in cell length was observed. The chromosome positioning occurred even under inhibition of the initiation of chromosome replication and under inactivation of DNA gyrase. The chromosome positioning presumably requires new synthesis of a particular protein(s) or translation itself.

MeSH Terms
Chloramphenicol/pharmacology Chromosomes, Bacterial/physiology DNA Replication DNA Topoisomerases, Type II/physiology Escherichia coli/drug effects,genetics Protein Biosynthesis Rifampin/pharmacology
Chemicals
Chloramphenicol DNA Topoisomerases, Type II Rifampin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hiraga S
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Ogura T
Niki H
Ichinose C
Mori H
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.1.31-39.1990
Published
1990-01-00
Pages
31-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208397
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.1.31-39.1990 PMC Full Text View journal details
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