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PMID: 2112747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TolC, an Escherichia coli outer membrane protein required for hemolysin secretion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 12 ·1990-06-00 ·Pages 4776-80

Wandersman C, Delepelaire P

Abstract

Secretion of Escherichia coli alpha-hemolysin into the medium does not require the cleavage of an N-terminal signal peptide. The specific secretion apparatus was shown to consist of two proteins, HlyB and HlyD, both located in the inner membrane and encoded by genes contiguous to the hemolysin structural gene (hlyA). It was proposed that these two proteins constitute a membrane-bound translocator for hemolysin [Mackman, N., Nicaud, J. M., Gray, L. & Holland, I. B. (1986) Curr. Top. Microbiol. Immunol. 125, 159-181]. We show here that an E. coli outer membrane protein, the TolC protein, encoded by a gene not located in the hly cluster, is specifically required for hemolysin secretion. This result suggests that an outer membrane protein might be a component of the secretion apparatus allowing a specific interaction between the inner and the outer membrane.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Cell Membrane/metabolism Coliphages/genetics Escherichia coli/genetics,metabolism Genotype Hemolysin Proteins/genetics,metabolism Mutation Phenotype Plasmids beta-Galactosidase/genetics beta-Lactamases/genetics
Chemicals
Bacterial Outer Membrane Proteins Hemolysin Proteins beta-Galactosidase beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wandersman C
Unité de Génétique Moléculaire, URA 1149 du Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Delepelaire P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.12.4776
Published
1990-06-00
Pages
4776-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54200
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.12.4776 PMC Full Text View journal details
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