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PMID: 1372386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A collection of mRNA species that are inducible in the RAW 264.7 mouse macrophage cell line by gamma interferon and other agents.

Molecular and cellular biology ·Vol. 12 ·No. 4 ·1992-04-00 ·Pages 1535-45

Farber JM

Abstract

To identify genes induced during macrophage activation, a cDNA library was prepared from cultures of the RAW 264.7 mouse macrophage cell line that had been treated with conditioned medium from mitogen-stimulated spleen cells, and the cDNA library was screened by differential plaque hybridization. Eleven cDNA clones, designated CRG-1 through CRG-11, corresponding to mRNA species inducible in RAW 264.7 cells by the spleen cell conditioned medium, were isolated. Inductions were not blocked by cycloheximide. All of the mRNAs were inducible by gamma interferon, and some were also inducible by alpha and beta interferons, by lipopolysaccharide, by phorbol 12-myristate 13-acetate, and by the calcium ionophore A23187. Sequencing of the cDNAs revealed that CRG-1, CRG-3, and CRG-5 are cDNAs of recently identified transcription factors IRF-1, zif/268, and LRF-1 respectively. As previously reported, CRG-2 and CRG-10 (MIG) encode new members of the platelet factor 4 family of cytokines. CRG-6 corresponds to a new member of a family of interferon-inducible genes clustered on mouse chromosome 1, CRG-9 corresponds to a prostaglandin synthase homolog, CRG-8 corresponds to beta 2-microglobulin, and CRG-4 corresponds to metallothionein II. CRG-11 contains sequences of a truncated L1Md repetitive element as well as nonrepetitive sequences. The nonrepetitive sequence of CRG-11 as well as the sequences of CRG-7 are not closely related to published sequences. The CRG genes and proteins are of interest because of their involvement in macrophage activation, because of their roles as mediators of the effects of gamma interferon and other pleiotropic agents, and because of their usefulness as tools for studying the signal pathways through which gamma interferon and other inducers exert their effects on gene and protein expression.

Related Genes
CRG-1 CRG-10 CRG-11 CRG-2 CRG-3 CRG-4 CRG-5 CRG-6 CRG-7 CRG-8 CRG-9
MeSH Terms
Animals Calcimycin/pharmacology Cell Division/drug effects Cell Line Chromosome Mapping Cloning, Molecular Culture Media/pharmacology Cytokines/pharmacology Gene Expression Regulation Interferon-gamma/pharmacology Interferons/pharmacology Lipopolysaccharides Macrophages/drug effects,metabolism Metallothionein/genetics Mice Prostaglandin-Endoperoxide Synthases/genetics RNA, Messenger/drug effects Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/genetics beta 2-Microglobulin/genetics
Chemicals
Culture Media Cytokines Lipopolysaccharides RNA, Messenger Transcription Factors beta 2-Microglobulin Calcimycin Interferon-gamma Interferons Metallothionein Prostaglandin-Endoperoxide Synthases Tetradecanoylphorbol Acetate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Farber J M
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.4.1535-1545.1992
Published
1992-04-00
Pages
1535-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369595
Subset
IM
Grants
NCI NIH HHS · CA-48059 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.4.1535-1545.1992 PMC Full Text View journal details
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