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PMID: 11892816 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential regulation of HLA-DR mRNAs and cell surface antigens by interferon.

The EMBO journal ·Vol. 2 ·No. 9 ·1983-00-00 ·Pages 1585-9

Rosa F, Hatat D, Abadie A, Wallach D, Revel M, Fellous M

Abstract

Human interferons-alpha, -beta and -gamma enhance HLA-DR mRNAs in all the human lymphoblastoid and melanoma cell lines studied. The increase concerns both alpha and beta chain mRNAs. Moreover, we show that immune interferon-gamma preferentially enhances class II MHC mRNA. This effect of IFN-gamma on the synthesis of alpha and beta HLA-DR chains has been also analysed by immunoprecipitation. It is abolished by a monoclonal antibody directed against human IFN-gamma. The effect of interferon on the cell surface level of HLA-DR molecules does not always correspond to the enhancement of HLA-DR mRNA. Our experiments suggest that this discrepancy between the enhancement of HLA-DR mRNA and cell surface antigen might be due to a constitutively high level of the corresponding antigens on several of the human cells studied.

MeSH Terms

B-Lymphocytes/drug effects,immunology Cell Line Gene Expression Regulation/drug effects HLA-A Antigens/metabolism HLA-B Antigens/metabolism HLA-C Antigens/metabolism HLA-DR Antigens/genetics,metabolism Humans Interferon-alpha/pharmacology Interferon-beta/pharmacology Interferon-gamma/pharmacology Interferons/pharmacology Melanoma/immunology RNA, Messenger/genetics

Chemicals

HLA-A Antigens HLA-B Antigens HLA-C Antigens HLA-DR Antigens Interferon-alpha RNA, Messenger Interferon-beta Interferon-gamma Interferons

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Rosa F

Immunogénétique Humaine, Institut Pasteur, Université Paris VII, France.

Hatat D

Abadie A

Wallach D

Revel M

Fellous M

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

1585-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555327

Subset

External Links

PubMed source PMC Full Text View journal details

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Differential regulation of HLA-DR mRNAs and cell surface antigens by interferon.

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