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PMID: 1321411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The AT-rich tract of the SV40 ori core: negative synergism and specific recognition by single stranded and duplex DNA binding proteins.

Nucleic acids research ·Vol. 20 ·No. 13 ·1992-07-11 ·Pages 3333-9

Galli I, Iguchi-Ariga SM, Ariga H

Abstract

The SV40 origin of replication comprises a run of thymine and adenine residues. Integrity of this AT-rich sequence is known to be essential for replication. We set out to study whether or not these elements can work synergistically to sustain replication. Quite surprisingly, additional copies of the AT stretch linked to a functional SV40 ori core dramatically reduce its replication in Cosl cells, probably by creating some physical block. Interestingly, the same inhibiting effect can be observed with the addition in cis of the yeast ARS consensus, which is homologous to the SV40 AT stretch. This modulation is possibly due to the action of cellular factors that recognize either of the two sequences. In fact, we demonstrate the existence of factor(s) in Cosl crude nuclear extracts that in vitro can specifically bind to either of them. Moreover, we show that these sequence-specific factor(s) (MW about 50 kDa), named SOAP, recognize both single (T-rich strand) and double stranded forms of the AT tracts. Binding to single stranded AT stretches can be specifically inhibited by the corresponding duplex form, but not vice versa.

MeSH Terms
Base Sequence Binding Sites/genetics DNA Replication/genetics DNA, Single-Stranded/genetics,metabolism DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism Molecular Sequence Data Oligodeoxyribonucleotides/genetics,metabolism Plasmids/genetics Simian virus 40/genetics Viral Proteins/metabolism
Chemicals
DNA, Single-Stranded DNA, Viral DNA-Binding Proteins Oligodeoxyribonucleotides Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galli I
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Iguchi-Ariga S M
Ariga H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.13.3333
Published
1992-07-11
Pages
3333-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312485
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.13.3333 PMC Full Text View journal details
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