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PMID: 11466295 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.

Journal of bacteriology ·Vol. 183 ·No. 16 ·2001-08-00 ·Pages 4905-9

McQuade RS, Comella N, Grossman AD

Abstract

A family of 11 phosphatases can help to modulate the activity of response regulator proteins in Bacillus subtilis. Downstream of seven of the rap (phosphatase) genes are phr genes, encoding secreted peptides that function as phosphatase regulators. By using fusions to lacZ and primer extension analysis, we found that six of the seven phr genes are controlled by the alternate sigma factor sigma-H. These results expand the potential of sigma-H to contribute to the output of several response regulators by controlling expression of inhibitors of phosphatases.

MeSH Terms
Bacillus subtilis/genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence Binding Sites Consensus Sequence DNA Primers DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genotype Molecular Sequence Data Multigene Family Phosphoric Monoester Hydrolases/genetics Promoter Regions, Genetic RNA, Messenger/genetics Recombinant Fusion Proteins Sequence Alignment Sequence Homology, Nucleic Acid Sigma Factor/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins DNA Primers DNA-Binding Proteins RNA, Messenger Recombinant Fusion Proteins Sigma Factor Phosphoric Monoester Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McQuade R S
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Comella N
Grossman A D
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43 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.16.4905-4909.2001
Published
2001-08-00
Pages
4905-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC99546
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050895 · United States
NIGMS NIH HHS · GM50895 · United States
External Links
PubMed source DOI: 10.1128/JB.183.16.4905-4909.2001 PMC Full Text View journal details
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