Home LiteratureArticle Details
PMID: 2507521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis.

Journal of bacteriology ·Vol. 171 ·No. 10 ·1989-10-00 ·Pages 5347-53

Nakano MM, Zuber P

Abstract

A Tn917 insertion mutation srfB impairs the production of the lipopeptide antibiotic surfactin in Bacillus subtilis. srfB is located between aroG and ald in the B. subtilis genome, as determined by phage PBS1 transduction mapping, and is not linked to the previously described surfactin loci sfp or srfA. A srfB mutant was found to be also deficient in the establishment of competence. SP beta phage-mediated complementation analysis showed that both competence and surfactin production were restored in the srfB mutant by a single DNA fragment of 1.5 kilobase pairs. The sequence of the complementing DNA revealed that the srfB gene is comA, an early competence gene which codes for a product similar to that of the activator class of bacterial two-component regulatory systems. The srfB mutation impaired the expression of a srfA-lacZ fusion, suggesting that surfactin production is positively regulated at the transcriptional level by the srfB (comA) gene product.

MeSH Terms
Bacillus subtilis/genetics Bacterial Proteins/biosynthesis,genetics Base Sequence Cloning, Molecular DNA-Binding Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Genetic Complementation Test Lipopeptides Molecular Sequence Data Mutation Peptides, Cyclic Recombinant Fusion Proteins/genetics Restriction Mapping
Chemicals
Bacterial Proteins DNA-Binding Proteins Lipopeptides Peptides, Cyclic Recombinant Fusion Proteins comB protein, Haemophilus influenzae surfactin peptide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nakano M M
Department of Botany and Microbiology, Oklahoma State University, Stillwater 74078.
Zuber P
References (34)
34 references, click to expand
  1. Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants.
    J Bacteriol. 1989 Oct;171(10):5362-75 PMID: 2507523
  2. Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis.
    J Bacteriol. 1987 Jul;169(7):3104-9 PMID: 3036770
  3. The peptide antibiotics of Bacillus: chemistry, biogenesis, and possible functions.
    Bacteriol Rev. 1977 Jun;41(2):449-74 PMID: 70202
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400.
    Plasmid. 1979 Jan;2(1):48-58 PMID: 112601
  6. Osmoregulation of gene expression. I. DNA sequence of the ompR gene of the ompB operon of Escherichia coli and characterization of its gene product.
    J Biol Chem. 1982 Nov 25;257(22):13685-91 PMID: 6292199
  7. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  8. Genetic transposition and insertional mutagenesis in Bacillus subtilis with Streptococcus faecalis transposon Tn917.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2305-9 PMID: 6300908
  9. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.
    Cell. 1983 Nov;35(1):275-83 PMID: 6414720
  10. Isolation and characterization of Bacillus subtilis mutants altered in competence.
    J Bacteriol. 1984 Jan;157(1):152-7 PMID: 6317647
  11. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions.
    Mol Gen Genet. 1984;195(3):424-33 PMID: 6088944
  12. Characterization of the spo0A locus and its deduced product.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2647-51 PMID: 3157992
  13. Revised genetic linkage map of Bacillus subtilis.
    Microbiol Rev. 1985 Jun;49(2):158-79 PMID: 2989674
  14. Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7260-4 PMID: 2997779
  15. Expression of competence genes in Bacillus subtilis.
    J Bacteriol. 1987 Jul;169(7):3110-7 PMID: 3110135
  16. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  17. Biosynthesis of peptide antibiotics.
    Annu Rev Microbiol. 1987;41:259-89 PMID: 3318671
  18. Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332.
    FEBS Lett. 1988 Apr 11;231(1):107-10 PMID: 3129307
  19. Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.
    J Bacteriol. 1988 Jun;170(6):2742-8 PMID: 3131310
  20. Deduced polypeptides encoded by the Bacillus subtilis sacU locus share homology with two-component sensor-regulator systems.
    J Bacteriol. 1988 Nov;170(11):5093-101 PMID: 3141377
  21. Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systems.
    J Bacteriol. 1988 Nov;170(11):5102-9 PMID: 3141378
  22. Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis.
    J Bacteriol. 1988 Dec;170(12):5662-8 PMID: 2848009
  23. Cloning and characterization of the regulatory Bacillus subtilis competence genes comA and comB.
    J Bacteriol. 1989 Oct;171(10):5354-61 PMID: 2507522
  24. DNA-damage-inducible (din) loci are transcriptionally activated in competent Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6201-5 PMID: 3929251
  25. Nitrogen fixation specific regulatory genes of Klebsiella pneumoniae and Rhizobium meliloti share homology with the general nitrogen regulatory gene ntrC of K. pneumoniae.
    Nucleic Acids Res. 1985 Jun 25;13(12):4539-55 PMID: 2989799
  26. Construction and properties of Tn917-lac, a transposon derivative that mediates transcriptional gene fusions in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):140-4 PMID: 3001720
  27. Identification of the pleiotropic sacQ gene of Bacillus subtilis.
    J Bacteriol. 1986 Apr;166(1):113-9 PMID: 3007431
  28. Chromosomal insertions of Tn917 in Bacillus subtilis.
    J Bacteriol. 1986 Aug;167(2):530-4 PMID: 3015878
  29. Growth phase and ompR regulation of transcription of microcin B17 genes.
    J Bacteriol. 1986 Sep;167(3):1058-65 PMID: 3017916
  30. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
    J Mol Biol. 1986 Oct 20;191(4):615-24 PMID: 3100810
  31. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
    J Bacteriol. 1987 May;169(5):2223-30 PMID: 2437099
  32. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.
    Cell. 1987 Jun 5;49(5):579-81 PMID: 3555840
  33. Relationship between aconitase gene expression and sporulation in Bacillus subtilis.
    J Bacteriol. 1987 Jul;169(7):3068-75 PMID: 3110134
  34. Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation.
    Biochem Biophys Res Commun. 1968 May 10;31(3):488-94 PMID: 4968234
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.10.5347-5353.1989
Published
1989-10-00
Pages
5347-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210372
Subset
IM
Grants
NIGMS NIH HHS · GM39479-01 · United States
External Links
PubMed source DOI: 10.1128/jb.171.10.5347-5353.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2